Stem cells, engineering and FACS

Biofabrication and Tissue Engineering Facility

The Biofabrication and Tissue Engineering Facility (Biofab) offers a diverse range of micro and nanostructuring techniques for a wide scope of applications in the fields of life-science and medicine. The Biofab aims to bridge the gap between engineering and biology at Karolinska Institutet and to provide common grounds with respect to suitable material of use of modular design and tunable structures for biomedical purposes.  This facility is available through the KI contact portal for core facilities in iLab.

CRISPR Functional Genomics

The CRISPR Functional Genomics facility provides affordable access to high throughput functional genomic screens using the CRISPR/Cas9 system in cell lines. Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands to tens of thousands of genes for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation).

They also offer generation of stable Cas9-expressing lines in users’ cells of interest. This facility is available through the KI contact portal for core facilities in iLab (under the name CRISPR Center Sweden).

Developmental Tissue Bank

The Developmental Tissue Bank provides research groups with defined human embryonic and fetal tissue (“prenatal tissue”), derived from routine abortions, through a collaboration with various clinics. After the tissue is transferred to the facility, we determine the developmental stage, identify the retrieved tissue, perform any necessary dissection, and process the tissue as agreed with the user. This facility is available through the KI contact portal for core facilities in iLab.

FACS Biomedicum

The Biomedicum flow cytometry core facility (BFC) is a shared resource laboratory that will provide researchers with the capability of performing a wide range of flow cytometry experiments. This facility is available through the KI contact portal for core facilities in iLab.

iPS Core Facility

The reprogramming facility provides a reprogramming service to supply scientists with human induced pluripotent stem (iPS) cells. Patient-specific iPS cell lines opens up unique opportunities to in vitro study and create cellular models of human disease. The facility reprograms human fibroblast cells using non-modified RNAs (NM-RNAS), which is a non-integrating method. This facility is available through the KI contact portal for core facilities in iLab.

Karolinska Genome Engineering

The Karolinska Genome Engineering Facility (KGE) are specialists in CRISPR/Cas technology, and it is their mission to facilitate and accelerate your research by providing flexible and affordable gene-editing services in cells. Your cell type, your edit – from strategy design to delivery of a cell pool with quantified allele frequency. KGE is a branch of the CRISPR Functional Genomics, a national SciLifeLab facility (see above), and together these facilities form CRISPR Center Sweden (CriCS). This facility is available through the KI contact portal for core facilities in iLab (under the name CRISPR Center Sweden).

Karolinska Institutet Stem Cell Organoid

The main goal of Karolinska Institutet Stem Cell Organoid (KISCO) is to assist researchers, mainly within the university and in Sweden, to harbour the potential of pluripotent stem cells (PSCs) in studying complex human disease mechanisms. A key tool offered and that will be continuously developed is the derivation and use of in vitro organoid systems of mouse and human stem cell origin. KISCO is unique in offering these services as a core facility in Sweden. This facility is available through the KI contact portal for core facilities in iLab.

MedH Flow Cytometry Facility

The Flow Cytometry Facility at the Department of Medicine, Huddinge (South Campus) offers a broad range of high quality services including assistance with experimental design, training for independent users, and operator-assisted services for cell analysis and cell sorting. This facility is available through the KI contact portal for core facilities in iLab.

VirusTech Core Facility

Genetically engineered viruses provide powerful tools for introducing desired genes in cells. To facilitate the use of this state-of-art methodology, the VirusTech core facility at KI's Department of Medical Biochemistry and Biophysics specializes in the production, purification and concentration of such virus particles. This facility is available through the KI contact portal for core facilities in iLab (under the name Common Virus Facility). 

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