Hormone signalling and non-coding RNAs in cancer - Cecilia Williams

Our research focuses on understanding key molecular mechanisms in cancer. Each cancer is unique and there is an urgent need for precision medicine, diagnostic tools and novel treatment approaches to reduce cancer mortality. We use a combination of large-scale omics together with focused mechanistic experiments and in vivo studies.

bilder och grafer över tarmar och celler
Colorectal cancer (top left) is the 3rd leading cause of cancer deaths, and is more common in men compared to women (top right, from Zheng et al., BMC Cancer, 2019). We have shown that colitis accompanied by colon tumors reduces the diversity of the microbiome and that this is further reduced if intestinal ERβ is deleted (middle right, from Ibrahim et al., Int. J Cancer 2019). Omics data proposes the underlying mechanisms (bottom right, Cover, Molecular Endocrinology), and in vivo micrometastasis assay using zebrafish embryos show that expression of ERβ in colorectal cancer cells reduces the cells’ migratory capacity (bottom left, Nguyen-Vu et al., 2016).

Our goal is to understand critical cancer pathways so that we can suggest better cancer treatments and preventive approaches, as well as biomarkers, to be developed for clinical use.

Non-coding RNA molecules are RNAs that do not encode for proteins. The family includes microRNAs which regulate translation of target mRNAs and long non-coding RNAs (lncRNAs) which can have active functions such as 3D-RNA molecules. Both types can function as biomarkers or therapeutic targets. We study how non-coding RNAs are impacted by cell differentiation, hormone signalling and cancer, and their corresponding functions.

Oestrogene signalling and sex differences in cancer

Some cancers (such as colorectal and liver) are more common in men, and others (breast and thyroid) are more common in women. We explore how the hormone oestrogen influences this and, for example, increases the growth of breast cancer while protecting against colorectal cancer.

rutnät med åtta mikroskopibilder
Antibody validation (PPZ0506) and tissue-microarrays (TMA) of cancerous clinical tissues confirms expression of nuclear ERβ in (i–l) 3 out of 4 granulosa cell tumours, (m) one case of melanoma, and (p) one case of thyroid cancer. The remaining 16 cancer types were all negative, here exemplified by (n) colorectal cancer and (o) breast cancer. Brown: positive IHC staining; Blue: hematoxylin counter staining. Scale bar indicates 50 mm. Arrows indicate examples of cells with nuclear staining (from Andersson et al. Nature Communications 2017).

The effect of oestrogen is mediated by three oestrogen receptors: ERα, ERβ and GPER1. These are expressed to various extent in different tissues in both women and men. ERα and ERβ are ligand-activated nuclear receptors and are excellent therapeutic targets. GPER1 is a G protein-coupled transmembrane receptor with promising therapeutic potential. Using technologies like RNA-Seq, ChIP-Seq, microbiota analysis, and proteomics, along with cell models, tissue-specific knockouts and clinical samples, we explore how these three receptors, and their ligands such as dietary oestrogens or endocrine disruptors, impact cancer. We are interested in understanding how this contributes to sex differences in cancer incidences and outcomes, as well as the intricate connection with diet/obesity, inflammation and carcinogenesis. Our goal is to contribute to better biomarkers, therapeutics and preventive approaches.

Group members

Amena Archer

Annan grund

Selected publications

Sexual dimorphism in the incidence of human cancers.
Zheng D, Trynda J, Williams C, Vold JA, Nguyen JH, Harnois DM, et al
BMC Cancer 2019 Jul;19(1):684

A miR-206 regulated gene landscape enhances mammary epithelial differentiation.
Wang J, Aydoğdu E, Mukhopadhyay S, Helguero LA, Williams C
J. Cell. Physiol. 2019 Dec;234(12):22220-22233

Antibody Validation Strategy for Nuclear Receptors.
Katona B, Ibrahim A, Sundberg M, Williams C
Methods Mol. Biol. 2019 ;1966():79-99

Colitis-induced colorectal cancer and intestinal epithelial estrogen receptor beta impact gut microbiota diversity.
Ibrahim A, Hugerth LW, Hases L, Saxena A, Seifert M, Thomas Q, et al
Int. J. Cancer 2019 06;144(12):3086-3098

Regulation of sex hormone receptors in sexual dimorphism of human cancers.
Zheng D, Williams C, Vold JA, Nguyen JH, Harnois DM, Bagaria SP, et al
Cancer Lett. 2018 12;438():24-31

A Comprehensive Pan-Cancer Molecular Study of Gynecologic and Breast Cancers.
Berger AC, Korkut A, Kanchi RS, Hegde AM, Lenoir W, Liu W, et al
Cancer Cell 2018 04;33(4):690-705.e9

Insufficient antibody validation challenges oestrogen receptor beta research.
Andersson S, Sundberg M, Pristovsek N, Ibrahim A, Jonsson P, Katona B, et al
Nat Commun 2017 06;8():15840

Research Networks

  • Pan-Gynecologic Cancers Analysis Working Group, The Cancer Genome Atlas
  • VR Research environment Interdisciplinary research (QuantumSense 2018-2025)
  • American Association for Cancer Research (AACR)

Prizes/Awards

  • Career Development Award (VINNOVA/ Marie-Curie Actions GROWTH 291795, Cecilia Williams, 2014-2018)
  • National Cancer Institute / NIH USA (R01 CA172437, 2013-2019)