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["no.","REPORTING QUALITY"]
[null,"Test compound and controls"]
[1,"The chemical name or other identification, such as CAS-number, of the test compound was given."]
[2,"The purity of the test compound was stated or is traceable according to information given regarding manufacturer and lot/batch number. In case of mixtures, the composition of different constituents was stated."]
[3,"The vehicle was described."]
[4,"It was stated that a negative control group was included."]
[null,"Animal model and housing conditions"]
[5,"The animal model (species, strain, age or life stage and sex) was described."]
[6,"The method for individual identification of animals was described."]
[7,"The housing temperature was stated."]
[8,"The relative humidity was stated."]
[9,"The light-dark cycle was described."]
[10,"The number of animals per sex in each cage was stated."]
[11,"The cage materials were described."]
[12,"Any materials used for physical enrichment were described."]
[13,"Water bottle materials were described."]
[14,"The bedding material used was described."]
[15,"The type and source of feed were reported."]
[16,"The source of drinking water was reported."]
[null,"Dosing and administration of the test compound"]
[17,"The administered dose levels or concentrations were stated."]
[18,"The method for allocating animals to different treatments was stated."]
[19,"The total number of animals per dose group was stated."]
[20,"The route of administration was stated."]
[21,"The sex and age (or life stage) of the animals at start of dosing was stated or is obvious from the information given, e.g. “pregnant rats were used” is enough information that animals are female and sexually mature/adult."]
[22,"The frequency and duration of dosing/administration of the test compound was stated."]
[null,"Data collection and analysis"]
[23,"The test and/or analytical methods used were sufficiently described to allow for evaluation of the reliability of results."]
[24,"The method for allocating animals to different tests and measurements (e.g. tissue collection or evaluation of functional or behavioural endpoints) was stated."]
[25,"The sex, age and number of animals per dose group subjected to separate tests and measurements was stated. "]
[26,"The statistical methods and software used were described."]
[27,"The statistical unit, e.g. the individual or the litter, was stated."]
[28,"All results for the investigated endpoints were reported. The most critical results were presented in tables and figures, including description of variation and statistically significant results."]
[null,"Funding and competing interests"]
[29,"The funding sources for the study were stated."]
[30,"Any competing interests were disclosed or it was explicitly stated that the authors did not have any competing interests."]
[null,"Other"]
[31,"Was all information that is indispensable for evaluating the reliability of data given? This includes information on the test compound and controls, test system, study design or study performance."]
[null,"METHODOLOGICAL QUALITY"]
[null,"Test compound and controls"]
[1,"The test compound or mixture was unlikely to contain any impurities that may significantly have affected its toxicity. "]
[2,"An appropriate vehicle was used that is not expected to interfere with the absorption, distribution, metabolism, excretion or toxicity of the test compound."]
[3,"A concurrent negative control group was included."]
[null,"Animal model and housing conditions"]
[4,"A reliable and sensitive animal model was used for investigating the test compound and selected endpoints."]
[5,"Animals were individually identified."]
[6,"Housing conditions (temperature, relative humidity, light-dark cycle) were appropriate for the study type and animal model. "]
[7,"The number of animals per sex in each cage were appropriate for the study type and animal model."]
[8,"The test system was unlikely to contain contaminants that could affect study results, such as organic pollutants, pesticide residues, heavy metals, and mycotoxins, as well as phytoestrogens."]
[null,"Dosing and administration of the test compound"]
[9,"The allocation of animals to different treatments was randomized."]
[10,"The route of administration was appropriate and not likely to interfere with the study results."]
[11,"The timing and duration of administration were appropriate for investigating the included endpoints."]
[12,"The frequency of administration was appropriate for investigating the included endpoints. "]
[null,"Data collection and analysis"]
[13,"The allocation of animals to different tests and measurements was randomized."]
[14,"Reliable and sensitive test methods were used for investigating the selected endpoints."]
[15,"Measurements were collected at suitable time points in order to generate sensitive, valid and reliable data."]
[16,"A sufficient number of animals per dose group were subjected to separate tests/data collection/measurements to generate reliable and valid results."]
[17,"The statistical methods have been clearly described and do not seem inappropriate, unusual or unfamiliar. "]
[null,"Other"]
[18,"Are there any other aspects of study design, performance or reporting that influence reliability? (Comment in free text.)"]
[null,"RELEVANCE"]
[1,"The identity of the tested substance."]
[2,"The animal model used."]
[3,"The endpoint studied. "]
[4,"The route of administration. "]
[5,"The dose levels and resulting tissue levels. "]