Environmental Factors and Health seminar
Seminars organised by the doctoral programme Environmental Factors and Health.
Ulrika Carlander - PBPK models for inhaled nanoparticles
This presentation will be about how we built a PBPK model for inhaled cerium oxide nanoparticles in rats and some experience related to the work. The model describes how inhaled cerium nanoparticles are absorbed, distributed and cleared from the lung as a function of time. When cerium oxide is inhaled, the nanoparticles will deposit in the respiratory system. The respiratory system can be divided into three parts, the upper respiratory tract, tracheabronchial region and alveolar region. The fraction of deposition in the three regions will depend on the properties of cerium oxide, the exposure conditions and physiological and anatomical properties of the rats. In the tracheabronchial region nanoparticles are cleared via mucus escalator clearance and transport to gastrointestinal tract. In the alveolar region nanoparticles are cleared either by translocation into the blood or lymphatic system or by uptake into alveolar macrophages for further transport to tracheabronchial region and transport to gastrointestinal tract. The model describes the biokinetic reasonable and we learnt a lot about parameters that influences the outcome as breathing volume and frequency, cerium dioxide size estimation, fur contamination.
For you who are interested to read a little bit about inhalation about nanoparticles.
Snipes MB1.Crit Rev Toxicol. 1989;20(3):175-211. Long-term retention and clearance of particles inhaled by mammalian species.
Kreyling WG1, Semmler-Behnke M, Takenaka S, Möller W. Acc Chem Res. 2013 Mar 19;46(3):714-22 Differences in the biokinetics of inhaled nano- versus micrometer-sized particles.
Emma Åkerlund - Can nickel nanoparticles induce epithelial to mesenchymal transition in epithelial lung cells?
Epithelial to mesenchymal transition is an important mechanism for cancer cells to form metastasis. Nickel is a carcinogen category 2 and exposure to nickel compounds by inhalation are known to be a risk for developing lung cancer. Yet, the mechanisms for nickel to induce lung cancer are not completely elucidated. Many studies includes results from exposures of high doses of nickel compounds. A previous study showed that a relatively high dose of soluble nickel can induce EMT in lung cells. To closer mimic the reality of lung exposure to nickel lower doses of exposure are used in this study. The overall aim of the study is to investigate the ability of nickel nanoparticles, in low concentrations, to induce EMT in an in vitro model using lung cells.