Shared facilities and processes in Biomedicum

The FM organization actively contributes to improving the environment for core business performance in order to achieve business goals and visions. FM provides coordination, initiation, monitoring and performance of the shared facilities and features in Biomedicum.

Chemicals

Barcodes and purchases of chemicals

A barcode system for identification and registration of chemicals will increase the efficiency of handling chemicals and improve the chemical safety.

All chemicals in Biomedicum will have a barcode with information about name, CAS number, quantity and storage location. This provides a continuously updated list in KLARA of all chemicals, for instance information of storage location, which is extremely important in case of fire or an accident.

All chemicals must be labelled with a barcode before they are relocated. All storage spaces in Biomedicum will be labelled with a barcode, which means that relocated chemicals can quickly be registered at a new storage location.

If chemicals are to be stored in other places, please contact FM Helpdesk for help with generating a new barcode.

When purchasing new chemicals, the user places an order in KLARA's purchase module, which also indicates the storage location. A purchaser at FM, will regularly compile all orders and send them to the respective supplier. Upon delivery, the chemicals labelled with a barcode prior transported to the user. 

The purchase of chemicals via KLARA will be available as from October 1, 2018. In the meantime, you may order your chemicals in the ordinary way. Please note that the chemicals you order will upon arrival need barcodes. You may print them yourself or contact FM's help desk for assistance.

Common facilities/labs

Common virus facilities BSL-2 (D0411 & D0413)

The virus common facility aims to provide a suitable, safe and up-to-date virus work laboratory.

This facility is equipped with all necessary tools/equipment to perform virus work including production, purification, concentration and titration.

The equipment in the facility can be booked to perform virus based experiments on a price-per-hour basis (to be determined).

Please observe that access to the facility is restricted. Permit notification of GMM, mandatory introduction and booking are required.

Infoposter Common Virus Facilities in Biomedicum (BSL-2) (pdf)

Contact person:

Albert Blanchart Aguado
E-mail: Virusfacility@ki.se

BSL-3

 

Clean room / Clean lab

 

Dark Room

On floor 2, room B0210 and room B0213

More information will be available shortly.

Radioactive facility

In Biomedicum there will be three common facilities for work with radioactive material.

Richard Odh, Security coordinator at KI, is currently coordinating the radioactive facilites regarding what instruments to keep and different work processes. He will contact those of you who carry a valid license in order to give you a proper introduction to the facilites.

For questions, contact:

Security coordinator

Richard Odh

Phone: +46-(0)8-524 861 54
Organizational unit: Miljö och säkerhet
E-mail: richard.odh@ki.se

Room Isotopes
Floor 5 room No C0511 Non extensive work. Low activity.
Radiotoxity class C and D isotopes.
Floor 7 room No D0711 Non extensive work. Moderate activity.
Radiotoxity class C and D isotopes.
Floor 9 room No D0911 Extensive work. High activity.
Radiotoxity class B and C isotopes, e.g. 125I.

All personnel who carry out work with radionuclides must in accordance with regulations set by the Swedish Radiation Safety Authority (SSM):

  • participate in a radiation safety lecture held by the employer.
  • have knowledge about local radiation protection rules as well as knowledge on risks from radiation. 
  • participate in local introduction to the facilities in Biomedicum.

The lecture in Radiation protection is held by the Department of Nuclear Medicine, Karolinska University Hospital and needs to be repeated every 5th year.

Strålsäkerhetsutbildning för nuklearmedicin och labverksamhet med öppna strålkällor (course website)

License

Core facilities

Biomedicum Flow Core (BFC)

The Biomedicum flow cytometry core facility (BFC) will be a comprehensive resource, equipped with instruments providing researchers with the capability of performing a wide range of flow cytometry experiments.

The facility will start functioning from the middle of May, 2018.

CLICK

 

Histology

The Histo-core has a number of cryostats, microtomes, dehydration machines and embedding platforms. It will operate on a self-service mode, i.e., the researchers can use the equipment by themselves on a price-per-hour scheme (the price will be defined shortly).

Contact person:

Senior researcher

Andrei Chagin

Phone: +46-(0)8-524 879 41
Organizational unit: Chagin Andrei group - Bone and Cartilage Physiology
E-mail: andrei.chagin@ki.se

VirusTech core (D0415)

The VirusTech core at Karolinska Institutet specializes in the production, purification and concentration of viruses including Lentiviruses (LVs), recombinant Adeno Associated Virus (rAAVs) and Gamma-retroviruses (RVs). Its primary goal is to help fellow researchers to overcome the challenges of using virus-based gene transfer into host cells, both in cultured cells and in whole organisms, as well as investigate the function of genetic programs, and/or specific cell classes, in the development, disease and cellular processes.

For more detailed information about services, fees and inquiries please visit KI´s VirusTech core facility.

Please observe that access to the facility is restricted to the virus core members only.

Infoposter VirusTech core facility in Biomedicum (pdf)

Contact person:

Laboratory coordinator

Albert Blanchart Aguado

Organizational unit: Andersson, Emma
E-mail: Albert.Blanchart-Aguado@ki.se

Handling of gas

Access to this room is restricted. Contact FM if you need access to the  premises.

Carbon dioxide (CO2), Nitrogen (N2), Oxygen (O2) and Carbogen (CO2 + O2) are gases that will be centrally provided in Biomedicum.

Other/special gases will be delivered in gas flasks to the gas flask storage room in your quarter. From this storage room, there will be tubings to your lab bench so that correct gas is delivered.

When a gas flask is about to run empty, a message will automatically be sent to FM, who will replace the empty bottle with a new one.

Contact FM Helpdesk for more information.

Lab coats

Lab coats to be autoclaved

  • If you need clean but autoclaved labcoats, send a ticket via FM Helpdesk to order autoclaved lab coats.
  • If you have a contaminated labcoat that needs to be autoclaved (decontaminated) prior to being washed, place it in a autoclave bag and label with name, quarter and the date.

label for items to be autoclaved (Biomedicum)

Until Karolinska Institutet signs a frame agreement with a supplier of laundry services, we will be using the lab coats that each department brings along.

  • Dirty lab coats will be placed in the waste management room and transported to floor 2 by FM-staff.
  • Clean lab coats will be placed in the room for clean lab glass ware on each floor.
  • Place a ticket using the category Dish washing and lab coats if you need autoclaved lab coats.

Lab glass

There is a central lab washing facility in Biomedicum.

  • Place used lab glass ware in the waste room in your quarter.
  • FM will collect all used lab glass ware from the quarters and transport it to the central dish washing unit on floor 2 on a daily basis.
  • Cleaned and, if needed, sterilized lab glass ware will be placed in the storage room (one per floor).
  • If you have group-specific lab glass ware, place it in a ‘container’ marked with your name and quarter. This will ensure that you will receive the same glass ware.

Follow special instructions if the lab glass ware is contaminated and needs to be autoclaved prior to dish washing. In the lab where the lab glass is used, place the contaminated lab glass in an autoclave bag and seal with autoclave tape. Only then can the bag be placed in the container marked ‘To be autoclaved’ in the waste room. 

Items to be autoclaved

Pipette tips, tubes, tools, solutions, etc. to be autoclaved

  • Place item in an autoclave bag
  • label with name, quarter and the date when you need it.
  • Put the bag in designated place in the room for clean lab glass ware (one per floor).

label for items to be autoclaved (Biomedicum)

If it is done prior to noon, it will be delivered back the same day.

Waste to be autoclaved

Solid:

  • Place the sealed bag with the waste in the red container in the waste room (one per quarter).
  • Label with following label. Important to mark if the waste contains any antibiotic or not.

label for items to be autoclaved_antibiotic (Biomedicum)

Liquid:

  • Place the container with liquid waste in the red container in the waste room (one per quarter).
  • Label with following label. Important to mark if the waste contains any antibiotic or not.
  • NB! It is the user's responsibility to ensure that the container used for the waste can be autoclaved. Otherwise, the autoclave procedure will be damaged.
  • If you don´t have a correct container, place the container with liquid waste in a yellow container for Sharp/Contagious instead.

label for items to be autoclaved_antibiotic (Biomedicum)

Liquid nitrogen and dry ice

Users register an errand in the Help desk, where the needed volume and delivery date are stated. FM will transport the liquid nitrogen via the prioritized elevator to the user.

Dry-ice (blocks and/or pellets) are kept available on level 2. Small quantities can be picked up by the users when needed. Larger quantities can be ordered separately and delivered to the desired internal address.

Low temperature freezer storage facility

On floor 2 there are several rooms for storage of low temperature freezers (-80C, -150C). In addition, there are about 20 freezers for storing samples in liquid nitrogen. You will be given access to the room in which you have your freezer.

For access to the facility with liquid nitrogen freezers, you need to attend and pass a special 'Liquid Nitrogen Safety Course'. For more information about the course, please contact Katrin Pütsep.

Narcotics and pharmaceuticals

Narcotics and pharmaceuticals can only be ordered by a ‘Läkemedelsföreståndare’ who has received a delegation from the Head of department.

When ordering narcotics and pharmaceuticals, the veterinarian must approve and initiate the order. The number of the current ethical permit of the preparation concerned must be clearly stated.

Room for handling CMR-classified chemicals

By law, the handling of CMR-classified chemicals must be done in a delimitated and defined area. This, in order to limit the number of people who might risk being exposed, and also, if you are pregnant or breastfeeding, you should be able to avoid these chemicals.

Therefore, special rooms are provided for the proper handling and storage of CMR-classified chemicals. 

The rooms should not be used as general chemical storage for all chemicals.

There are two ‘CMR-rooms’ per floor, which means that two quarters will have to share.

Supply Center

The Supply Center storage room in Biomedicum will be operative as from June 1, however you can continue to make purchases through KI’s e-purchasing system.

Ordered goods will be delivered to the quarter on a daily basis.

We have a supply of Ethanol in place in Biomedicum that can be ordered via FM Helpdesk. Make sure to mention your name, quarter, project number and zz-code on your order. FM will deliver directly to your quarter.

Water

In Biomedicum we have special taps for clean water that are placed in the room for apparatus. The water has the same or even higher quality than MilliQ-water.

It is not allowed to install old MilliQ-machines. This will ‘destroy’ the clean water.

If you need autoclaved water – use a pre-sterilized bottle designated for water only, that can be found in the room for clean lab glass ware. Fill it with clean water and follow the procedure described above for Items to be autoclaved.

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