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KIGene - genetic analyses at CMM

The former core facilities for DNA sequencing and genotyping at CMM L8:00, and the DNA sequence facility, KISeq, established at CMB 1992, merged year 2008 into the new core facility KIGene - genetic analyses at CMM. We are located at the Department of Molecular Medicine and Surgery, the Neurogenetics Unit, CMM L8:00, Karolinska University Hospital at Solna.

With experiences from two decades of running a core facility we have accumulated much knowledge and proficiency in how to perform services, superwise and support the users. Our vision is to provide a high quality, reliable, high efficiency and flexible core facility, aiming at providing the right solution and study set-up for the right project.

In order to meet the increasing demand on higher throughput and efficiency, as well as on cheaper analyses, our core-facility has introduced several options for gene expression analysis and the analysis of genetic variations including SNPs, microsatellite markers and insertion/deletion polymorphisms.

Our equipment can be used by those who have sufficient knowledge and experience. The facility provides start up theoretical and technical assistance. For some applications, we also offer full-service.

Our offer

  • Sanger sequencing services with turn-around time within the same day
  • Genotyping (SNP and fragment analysis).
  • Gene expression analysis.
  • Copy number variation
  • Rare target detection
  • microRNA profiling
  • Quantity and quality determination of RNA, DNA, protein and dye incorporation.
  • DNA methylation analysis
  • Basic molecular biology methods
  • Customer support

Flexible services

"Hands-on" service

The user receives a short introduction by experienced personnel regarding sample preparation and operation of the instruments. Each user reserves a date and time for occupying an instrument by a booking system.

"Hands-off" service

The user provides us with pre-made sequence or fragment analysis reactions and we are performing the analysis. The resulting files are transferred to each user by e-mail.


The user provides us with starting materials- as saliva, biopsies, DNA, oligonucleotides- and we are performing the analysis. The resulting files are transferred to each user by e-mail.

Instruments and applications

ABI 3730 PRISM® DNA Analyzers for:

  • DNA sequence analysis
  • fragment analysis

More information about ABI 3730 and price list (pdf)

Ion Proton Sequencer for:

  • Next Generation Sequencing

Please contact us for more information.

NanoString nCounter® FLEX analysis system for:

  • Gene expression analysis
  • Copy number variation
  • miRNA profiling

More information about the NanoString nCounter® system and price list (pdf)

QuantStudio 3D instrument for:

  • Absolute quantification of nucleic acids by digital PCR
  • Copy number variation
  • gene expression analysis
  • rare sequence detection

More information about the QuantStudio 3D system and price list (pdf)

QuantStudion 6 and 7 Flex (QS6 and 7 FLX) for:

  • real-time gene expression analysis
  • SNP analysis
  • allelic discrimination (end-point analysis)

More information about QuantStudion 6 and 7 Flex (QS6 and 7 FLX) and price list (pdf)

Pyromark Q96 ID and Pyrosequencing Assay Design Software

  • tri-plex SNP analysis
  • microsatellite scoring
  • haplotyping
  • CpG-methylation analysis
  • allelic frequency quantification
  • short sequence analysis

More information about Pyromark Q96 ID and price list (pdf)

NanoDropTM ND1000 Spectrophotometer

  • small volume quantity and quality determination of DNA, RNA and proteins

More information about NanoDropTM1000 and price list (pdf)

Agilent 2100 Bioanalyzer for

  • small volume quality determination of DNA and RNA

More information about Agilent 2100 Bioanalyzer and price list (pdf)

Order forms, Sanger DNA sequencing information and trouble shooting

Requirements for "full-service" Sanger DNA sequencing and primers stocked at KIGene (pdf)

Order form for full-service Sanger DNA sequencing (pdf, fill-in)

Order form for pre-made Sanger DNA sequencing reaction (pdf, fill-in)

Order form for pre-made fragment analysis samples (pdf, fill-in)

Information about Sanger DNA sequencing and trouble shooting (pdf)

Closing schedule 2019

  • May 31
  • June 7
  • November 1
  • December 23
  • December 27
  • December 30

Contact and organization

e-mail address:


Malin Alvehus

Telefon: 08-517 726 63
Enhet: Neurogenetik


Katarina Gell

Telefon: 08-517 726 63
Enhet: Neurogenetik

Senior lab manager

Annika Eriksson

Telefon: 08-517 726 63
Enhet: Neurogenetik

Scientific Director: Professor Martin Schalling