
Research description
Situated in the Swedish Medical Nanoscience Center, Richter-Dahlfors’ research group embraces scientists with expertise in microbiology/infection, neuroscience, and medicine as well as chemistry, organic- and microelectronics. Via extensive engagement in national and international multidisciplinary collaborations, we focus on defining the integrated pathophysiology of bacterial infections, and on generating novel technologies for neuromodulation.
Tissue microbiology reveals the integrated pathophysiology of infection: By applying a panel of intravital techniques (e.g. intravital 2-photon microscopy, amperometric biosensors), we study the real-time dynamics of the infection process within the organ at high resolution using uropathogenic E. coli-induced pyelonephritis as model.
Our work
- aids in establishing the emerging field ‘tissue microbiology’
- advances the development of biomimetic tools for complementary in vitro studies
- focuses on developing biosensors for hospital-acquired infections
Organic bioelectronics in nanomedicine: We recently demonstrated the use of conducting polymers for precise, non-convective, electrophoretic delivery of signal substances, such as ions and neurotransmitters. Analogously to the function of neurons, the device mimics the action potential causing release of chemical substances into the synaptic cleft. We currently focus on integrating this technology as a novel machine-to-brain interface and as an implantable neuromodulation technology.
Links
Publications
Organic Bioelectronic Tools for Biomedical Applications
ELECTRONICS 2015;4(4):879-908
Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier integrity
JOURNAL OF MATERIALS CHEMISTRY B 2015;3(25):4997-5000
Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds
Nanoscale 2015;7(44):18789-98
Bacterial nanoscale cultures for phenotypic multiplexed antibiotic susceptibility testing
Journal of clinical microbiology 2014;52(9):3310-7
Biomimetic interfaces reveal activation dynamics of C-reactive protein in local microenvironments
Advanced healthcare materials 2014;3(11):1733-8
Intravital two-photon imaging to understand bacterial infections of the mammalian host
Methods in molecular biology (Clifton, N.J.) 2014;1197():87-100
Polycaprolactone-thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
Journal of biomedical materials research. Part B, Applied biomaterials 2014;102(7):1553-61
Organic bioelectronics for electronic-to-chemical translation in modulation of neuronal signaling and machine-to-brain interfacing
Biochimica et biophysica acta 2013;1830(9):4334-44
Organic bioelectronics - novel applications in biomedicine. Preface
Biochimica et biophysica acta 2013;1830(9):4283-5
Intravital models of infection lay the foundation for tissue microbiology
FUTURE MICROBIOLOGY 2012;7(4):519-33
MULTIPHOTON MICROSCOPY APPLIED FOR REAL-TIME INTRAVITAL IMAGING OF BACTERIAL INFECTIONS IN VIVO
IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS: IMAGING LIVE CELLS IN HEALTH AND DISEASE 2012;:35-61
Swedish Medical Nanoscience Center at Karolinska Institutet
NANOTECHNOLOGY REVIEWS 2012;1(3):273-279
The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
Biomaterials 2012;33(19):4947-56
Tissue microbiology provides a coherent picture of infection
CURRENT OPINION IN MICROBIOLOGY 2012;15(1):15-22
Comparative tissue transcriptomics reveal prompt inter-organ communication in response to local bacterial kidney infection
BMC GENOMICS 2011;:123-
Electronic Control of Cell Detachment Using a Self-Doped Conducting Polymer
ADVANCED MATERIALS 2011;23(38):4403-8
Mechanical stimulation of epithelial cells using polypyrrole microactuators
LAB ON A CHIP 2011;11(19):3287-93
Organic bioelectronics in nanomedicine
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2011;1810(3):276-85
Special issue on "Nanotechnologies: Emerging applications in biomedicine"
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2011;1810(3):237-8
Uropathogenic Escherichia coli P and Type 1 Fimbriae Act in Synergy in a Living Host to Facilitate Renal Colonization Leading to Nephron Obstruction
PLOS PATHOGENS 2011;7(2):e1001298-
Ion bipolar junction transistors
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010;107(22):9929-32
Novel Innate Immune Functions Revealed by Dynamic, Real-Time Live Imaging of Bacterial Infections
CRITICAL REVIEWS IN IMMUNOLOGY 2010;30(2):107-17
Active Control of Epithelial Cell-Density Gradients Grown Along the Channel of an Organic Electrochemical Transistor
ADVANCED MATERIALS 2009;21(43):4379-82
Electrochemical modulation of epithelia formation using conducting polymers
BIOMATERIALS 2009;30(31):6257-64
Multiphoton imaging of host-pathogen interactions
Biotechnology journal 2009;4(6):804-11
Nano-fiber scaffold electrodes based on PEDOT for cell stimulation
SENSORS AND ACTUATORS B-CHEMICAL 2009;142(2):451-456
Omeprazole Antagonizes Virulence and Inflammation in Salmonella enterica-Infected RAW264.7 Cells
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 2009;53(6):2402-9
Organic electronics for precise delivery of neurotransmitters to modulate mammalian sensory function
NATURE MATERIALS 2009;8(9):742-6
Real-time live imaging to study bacterial infections in vivo
CURRENT OPINION IN MICROBIOLOGY 2009;12(1):31-6
Role of alpha-hemolysin induced Ca2+ signaling in the progression of uropathogenic E-coli infection within the urinary tract
FEBS JOURNAL 2009;:51-51
Translating Electronic Currents to Precise Acetylcholine-Induced Neuronal Signaling Using an Organic Electrophoretic Delivery Device
ADVANCED MATERIALS 2009;21(44):4442-+
A Bacterial Cytotoxin Identifies the RhoA Exchange Factor Net1 as a Key Effector in the Response to DNA Damage
PLOS ONE 2008;3(5):e2254-
Bacterial infection-mediated mucosal signalling induces local renal ischaemia as a defence against sepsis
CELLULAR MICROBIOLOGY 2008;10(10):1987-98
Electronically controlled pH gradients and proton oscillations
ORGANIC ELECTRONICS 2008;9(3):303-309
Electronic control of Ca2+ signalling in neuronal cells using an organic electronic ion pump
NATURE MATERIALS 2007;6(9):673-9
Organic bioelectronics
ADVANCED MATERIALS 2007;19(20):3201-3213
Progression of bacterial infections studied in real time - novel perspectives provided by multiphoton microscopy
CELLULAR MICROBIOLOGY 2007;9(10):2334-43
Real-time studies of the progression of bacterial infections and immediate tissue responses in live animals
CELLULAR MICROBIOLOGY 2007;9(2):413-24
Role of the lipopolysaccharide-CD14 complex for the activity of hemolysin from uropathogenic Escherichia coli
INFECTION AND IMMUNITY 2007;75(2):997-1004
A minimal generic model of bacteria-induced intracellular Ca2+ oscillations in epithelial cells
BIOPHYSICAL JOURNAL 2005;88(4):2976-81
Effects of the Escherichia coli toxin cytolysin A on mucosal immunostimulation via epithelial Ca2+ signalling and Toll-like receptor 4
CELLULAR MICROBIOLOGY 2005;7(6):779-88
Imaging Techniques for the Study of Escherichia coli and Salmonella Infections
EcoSal Plus 2005;1(2):-
Bacterial protein toxins and inflammation
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES 2003;35(9):628-31
Developmental aspects of Escherichia coli-induced innate responses in rat renal epithelial cells
PEDIATRIC RESEARCH 2003;54(4):536-41
Gastric mucosal recognition of Helicobacter pylori is independent of Toll-like receptor 4
JOURNAL OF INFECTIOUS DISEASES 2003;187(5):829-36
Helicobacter pylori infection induces interleukin-8 receptor expression in the human gastric epithelium
INFECTION AND IMMUNITY 2003;71(6):3357-60
Nanoscale features influence epithelial cell morphology and cytokine production
BIOMATERIALS 2003;24(20):3427-36
Structural requirements for TLR4-mediated LPS signalling: a biological role for LPS modifications
MICROBES AND INFECTION 2003;5(12):1057-63
Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin
CELL 2003;115(1):25-35
Dual effects of Escherichia coli alpha-hemolysin on rat renal proximal tubule cells
KIDNEY INTERNATIONAL 2002;62(6):2035-42
Expression and regulation of the pattern recognition receptors Toll-like receptor-2 and Toll-like receptor-4 in the human placenta
IMMUNOLOGY 2002;107(1):145-51
Host-pathogen interactions: Structure and function of pili
MOLECULAR CELLULAR MICROBIOLOGY 2002;:133-159
Identification of target tissue glycosphingolipid receptors for uropathogenic, F1C-fimbriated Escherichia coli and its role in mucosal inflammation
JOURNAL OF BIOLOGICAL CHEMISTRY 2002;277(20):18198-205
TLR4-dependent lipopolysaccharide signalling in epithelial cells is independent of extracellular protease activity
CELLULAR MICROBIOLOGY 2002;4(5):297-303
TLR4-dependent recognition of lipopolysaccharide by epithelial cells requires sCD14
CELLULAR MICROBIOLOGY 2002;4(8):493-501
Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells
JOURNAL OF EXPERIMENTAL MEDICINE 2002;195(5):559-70
Toxin-induced calcium oscillations: a novel strategy to affect gene regulation in target cells
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY 2002;291(6-7):511-5
Induction of innate immune responses by Escherichia coli and purified lipopolysaccharide correlate with organ- and cell-specific expression of Toll-like receptors within the human urinary tract
CELLULAR MICROBIOLOGY 2001;3(3):153-8
Receptor-mediated endocytosis in an apicomplexan parasite (Toxoplasma gondii)
EXPERIMENTAL PARASITOLOGY 2001;98(3):134-44
Uroepithelial cells require TLR4, CD14 and proteases for induction of proinflammatory responses when exposed to LPS
JOURNAL OF LEUKOCYTE BIOLOGY 2001;:65-66
alpha-Haemolysin of uropathogenic E-coli induces Ca2+ oscillations in renal epithelial cells
NATURE 2000;405(6787):694-7
Helicobacter pylori-infected human antral primary cell cultures: effect on gastrin cell function
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 1998;275(3):G393-401
Murine salmonellosis studied by confocal Microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo
JOURNAL OF EXPERIMENTAL MEDICINE 1997;186(4):569-80
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Presentations
Swedish Medical Nanoscience Center brochure
http://issuu.com/netdesign/docs/medicalnanoscience?e=1088764/1400888#search
Videos