Research seminar: RESOLFT super resolution microscopy - applications for neuroscience
RESOLFT super resolution microscopy: Applications for neuroscience
Research seminar with Ilaria Testa, PhD, Royal Institute of Technology & SciLifeLab, Stockholm
Date and time: Monday, May 25, @ 3:00 p.m.
Venue: Tellus seminar room, Dept of Neuroscience, KI, (Retzius Laboratory, 5th floor, corridor A:1)
Ilaria Testa, working with Stefan Hell at the Max Planck Institute in Göttingen, Germany, has developed the REversible Saturable OpticaL Fluorescence Transitions (RESOLFT) technique for work in organotypic brain cultures. RESOLFT, which is partly based on STED principles, is a super resolution imaging strategy that allows for high-speed imaging of nano-scale events where the resolution of conventional confocal microscopy techniques fail. Using this approach, Testa and colleagues have been able to visualize plastic remodeling in the dendritic spine followed over seconds-hours. Dr. Testa was recently recruited as a principal investigator to SciLife Lab where her laboratory will use super resolution microscopy techniques to address the synaptic basis for plasticity in the nervous system.
Nanoscopy of living brain slices with low light levels.
Testa et al., 2012. Neuron; 75(6):992-1000
Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.
Testa et al., 2015. Nano Lett. 2015; 15(1):103-6.
Host: Christian BrobergerContact person: Christian Broberger