Stem Cell & Organoid Core Facility
The Stem Cell & Organoid Core Facility (CMM-OrganoidCellStem) is located at CMM, Karolinska University Hospital, with the aim to accelerate research in the clinical applications of human stem cells by facilitating the generation and distribution of human pluripotent stem cells (hPSC), derived cells and organoids. HPSC include human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC).
The reprogramming of human somatic cells (for example, skin fibroblasts) to hiPSC has proven to be probably the major advance in stem cell research over the last years. Disease-specific iPSC is a powerful tool to study the mechanisms of disease and ultimately to develop new treatments.
Our facility offers the possibility of differentiate hPSC into different types of derived cells of the mesoderm, ectoderm and endoderm lineages. Our main focus is the production of organoids to advances translational research. Organoid models include three dimensional cell culture systems that closely resemble the in vivo organ or tissue. Organoids reproduce the complex spatial morphology of a tissue including relevant cell-cell and cell-matrix interactions. This is in contrast to traditional two dimensional cell culture models.
Our facility will also provide support for the maintenance and differentiation of established hPSC (both hESC and hiPSC) lines and training for researchers.
Depending on your requirements, the CMM-OrganoidCellStem operates on a fee-for-service basis or on a more collaborative basis. Contact us using the following form (LINK to download PDF).
Reprogramming of human fibroblasts using the episomal vector technology
This service, which takes 2-3 months to be completed, includes:
- Fibroblast isolation from human skin biopsies, expansion and freezing (3-5 vials).
- Reprogramming by nucleofection with the reprogramming episomal vectors.
- Picking and isolating iPSC colonies.
- Expansion of colonies and basic characterization (expression of pluripotency factors through RT-PCR and immunocytochemistry).
- Freezing hiPSC lines (2-6 vials per line).
- Mycoplasma testing
- OPTIONAL: Deeper characterization of hiPSC lines by NANOSTRING technology.
Maintenance and distribution of hiPSC and hESC lines
This service provides the possibility of obtaining hiPSC and hESC “ready to use” for the researcher. Maintenance and differentiation of hiPSC and hESC requires expertise and dedication, and we can assist in this daily task. The CMM-OrganoidCellStem facility can support researchers for the maintenance of their different hiPSC and hESC lines.
Generation of hiPSC and hESC derived cells
This service will meet the needs of each researcher, to provide different “ready to use” cell types derived from hiPSC or hESC. This service includes generation of several cell types such as embryoid bodies, neural rosettes, neural progenitors and different types of differentiated cells. We are open to meet the researcher needs for the adaptation of new protocols to generate different cell types in a collaborative effort.
Organoids generation for in vitro assays
An organoid is a miniaturized and simplified version of an organ produced in vitro. Organoids are grown in three dimensions and show realistic micro-anatomy. The in vitro organoid model has represented a major technological breakthrough that has already been established as an essential tool in many different areas including basic biology and clinical applications. The objective of this service is to assist investigators to use state-of-the-art technology of organoids to advance translational research. The CMM-OrganoidCellStem is adapting different protocols to offer different types of organoids. Please, contact us to discuss about the different possibilities.
A major advantage of the organoid technology is the ability to greatly expand both tissue-specific stem cells and their differentiated progeny from very limited amounts of starting material. This aspect facilitates tremendously the analyses of stem cell biology and provides a fantastic tool for in vitro disease modelling, generation of new tissue for regenerative purposes, and drug and genetic screenings.
Hands-on training option in basic hiPSC/hESC culture
For researchers with advanced cell culture experience. Basic techniques in hESC/iPSC culture including cell thawing, expansion, identification by morphology, manual and enzymatic passaging, freezing, and feeder-free culture will be covered.
Please contact us if you require custom services not listed above. We will do our best to accommodate your needs.
Carlos Villaescusa, PhD, Scientific Manager
Neurogenetics Unit | CMM L8:00 | Karolinska University Hospital | 17176 Stockholm | Sweden
firstname.lastname@example.org | Mobile phone: +46-702798755