External Biomedicum seminar: Quantifying transcription kinetics by single molecule imaging in living cells and embryos
Lecture by Professor J. Christof M. Gebhardt, Ulm University, Germany.
In a living organism, numerous inanimate biomolecules and their inherently stochastic interactions are precisely orchestrated and organized in space and time, together enabling the enigma of life. By using single molecule fluorescence microscopy we obtain an exceptionally detailed view on life processes in the natural environment of a live cell or embryo. Tracking individual molecules yields quantitative information such as reaction rate constants, diffusion coefficients and the spatial distribution and stoichiometry of cellular structures. Thus, we obtain information necessary for a deep understanding and modeling of life processes.
I will give a short overview over our imaging methodologies and then focus on recent findings regarding the dynamic cell-cycle dependent action of chromatin-structuring proteins, the implications of transcription factor - DNA interaction kinetics on the transcription process and on the activation of transcription in zebrafish development.
For more information or to meet the speaker please contact:
Annina Steinbach, MBB.Contact person: Annina Steinbach