Biophysics of stem cell and tissue growth

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Research in our group aims for a deeper understanding of the factors that control self-renewal in stem cells.

Self-renewal is the capacity of a stem cell to maintain a stem cell identity while realizing its potential to differentiate. Self-renewal necessarily also entails maintained proliferative capacity. Understanding the factors that control proliferation is essential when developing regenerative therapies but also therapies against self-renewing tumor initiating cells (cancer stem cells) that underlie tumor relapse and metastasis.

We aim to identify factors that:

  • induce the transition from quiescence to active proliferation in stem cells to allow tissue repair/engineering
  • factors that protect stem cells during radio-/chemotherapy and
  • factors that target the proliferative and metastatic capacity in cancer stem cells.

To this end we explore how biophysical mechanisms, involving membrane potential and electrochemical signaling, affect stem cell behavior and morphology associated with growth, proliferation and migration. We furthermore explore how photobiological and biophysical tools (e.g. optogenetics, ion channels) may integrate with these functions and aim to identify potential downstream effects on the cell intrinsic molecular clock and on metabolism.

We work with an experimental systems biology approach reaching from pharmacology, small molecule screens and genetic validations in stem cells, cancer stem cells and mouse and zebrafish models, extending to genomics (RNAseq) and a range of biophysical methods.

Group members

Michael Andäng  Research group leader
Cecilia Gardmo Associated
Helena Johard Associated
Petra Sekyrova Associated
Zuzana Sramkova Associated

Past group members:

Mia Niklasson, post-doc, present position senior researcher at Uppsala University. 


1. Membrane potential and ion fluxes in control of:

  • 1.1 the cell cycle in embryonic stem cells (Shaimaa Abdelhady).
  • 1.2 the cell cycle in neural stem cells (Anna Omelyanenko, Fei Gao).
  • 1.3 cell proliferation in glioma tumors and glioma cancer stem cells (Shermaine Wee).
  • 1.4 embryo development (Petra Sekyrova). 
  • 1.5 molecular clock oscillations (Anna Omelyanenko).

2 Cell cycle dependent gene expression - from transcription factors to ion channels - in:

  • 2.1 embryonic stem cells, induced pluripotent stem cells and cancer cells (Petra Sekyrova).
  • 2.2 neural stem cells.
  • 2.3 cancer stem cells (Shermaine Wee).
  • 2.4 interactions with Epithelial to Mesenchymal Transition in G2 cell cycle phase (Petra Sekyrova).

3. Light and magnetic field controlled gene expression and function:

  • 3.1 in control of basic biological mechanisms such as cell cycle and molecular clock oscillations (Petra Sekyrova, Anna Omelyanenko).
  • 3.2 reengineered to experimentally control gene expression (all members of the group).

Financial support


Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation.
Andäng M, Hjerling-Leffler J, Moliner A, Lundgren T, Castelo-Branco G, Nanou E, et al
Nature 2008 Jan;451(7177):460-4

Membrane-Depolarizing Channel Blockers Induce Selective Glioma Cell Death by Impairing Nutrient Transport and Unfolded Protein/Amino Acid Responses.
Niklasson M, Maddalo G, Sramkova Z, Mutlu E, Wee S, Sekyrova P, et al
Cancer Res. 2017 04;77(7):1741-1752

Endogenous GAB receptor activity suppresses glioma growth.
Blanchart A, Fernando R, Häring M, Assaife-Lopes N, Romanov R, Andäng M, et al
Oncogene 2017 02;36(6):777-786

ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner. Omelyanenko A, Sekyrova P, Andäng M. Springerplus. 2016 Jan 16;5:41.

Selective calcium sensitivity in immature glioma cancer stem cells.
Wee S, Niklasson M, Marinescu V, Segerman A, Schmidt L, Hermansson A, et al
PLoS ONE 2014 ;9(12):e115698

RETRACTED: Vulnerability of glioblastoma cells to catastrophic vacuolization and death induced by a small molecule.
Kitambi S, Toledo E, Usoskin D, Wee S, Harisankar A, Svensson R, et al
Cell 2014 04;157(2):313-328

Erg channel is critical in controlling cell volume during cell cycle in embryonic stem cells.
Abdelhady S, Kitambi S, Lundin V, Aufschnaiter R, Sekyrova P, Sinha I, et al
PLoS ONE 2013 ;8(8):e72409

Blebbing as a physical force in cancer EMT - parallels with mitosis.
Sekyrova P, Östblom J, Andäng M
Semin. Cancer Biol. 2012 Oct;22(5-6):369-73

Quiescence and γH2AX in neuroblastoma are regulated by ouabain/Na,K-ATPase.
Hiyoshi H, Abdelhady S, Segerström L, Sveinbjörnsson B, Nuriya M, Lundgren T, et al
Br. J. Cancer 2012 May;106(11):1807-15

Cell cycle restriction by histone H2AX limits proliferation of adult neural stem cells.
Fernando R, Eleuteri B, Abdelhady S, Nussenzweig A, Andäng M, Ernfors P
Proc. Natl. Acad. Sci. U.S.A. 2011 Apr;108(14):5837-42

Mouse embryonic stem cell-derived spheres with distinct neurogenic potentialsMoliner A, Enfors P, Ibáñez C, Andäng M. 
Stem Cells Dev. 2008 Apr;17(2):233-43.

Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation.
Andäng M, Hjerling-Leffler J, Moliner A, Lundgren T, Castelo-Branco G, Nanou E, et al
Nature 2008 Jan;451(7177):460-4

Contact us


Michael Andäng

Enhet: Andäng Michael grupp - Biofysisk stamcells- och vävnadstillväxt

PharmacologyStem cells