Our services to your research
The VirusTech Facility offers Lentiviruses, Gamma-retroviruses and Adeno Associated Viruses (AAV), each of which has different capabilities, weakness and strengths (see table below). If you have decided what type of viruses will suit your research project best, please continue reading Before starting a Project and Our viruses sections to find out more of our services, price lists and ordering.
|Packaging capacity (kB)||7 - 9||≈ 10||4 - 5|
|Transduction post-mitotic cells||Yes||No||Yes|
|Chance of oncogene activation||Yes||Yes||No|
The core facility also offers other services such as DNA preparation at different scales (mini, maxi and mega), as well as cloning of your inserts in our catalog of different expression vectors. We also offer consulting in order to help researchers to decide which type would fit their need for a specific project.
|Services||Prices (in SEK, as per plasmid)|
|Internal (KI users)||External|
|DNA mini prep||500||600|
|DNA Maxi prep (endo-free)||1500||3600|
|DNA Giga prep (endo-free)||3600||7600|
The capacity to efficiently transduce nondividing cells, shuttle large genetic payloads, and maintain stable long-term transgene expression are attributes that bring lentiviral vectors to the forefront of gene delivery vehicles for research.
Adeno Associated Viruses (AAVs)
AAV serotypes have shown different tissue tropisms in vivo, improved performance in terms of transduction efficiency, and fewer problems associated with humoral and/or cellular immunity.
The Gammaretrovirus is a sister genus to the lentivirus clade. Like the lentivirus, it is a member of the Orthoretrovirinae subfamily of the retrovirus family, they share common properties but the main difference between these two viruses is that Gammaretroviruses only infect dividing cells.