Monitoring human exposure to ultraviolet radiation - Dan Segerbäck
The work on UV has been focused on the analysis of thymidine dimer (T=T) in urine within the EU project ICEPURE. Quantification of T=T in urine samples collected before and after various outdoor activities showed that high levels were formed when spending one week at the island Tenerife, but not when skiing during one week in the Alps (limited skin exposure during such activities). In another setting people were experimentally exposed to full body UV radiation and skin biopsies and urine samples collected at different time points after exposure. The analyses of T=T showed that there was a correlation between T=T in the urine and in the skin and that morning urine samples well reflected T=T levels in 24 h urine samples. In other UV-related projects we have shown that there was no difference in urinary T=T levels between children and adults after spending 1-2 days on a beach in summer time Stockholm and that the amount of T=T was correlated to the UV dose. We also demonstrated that very high levels of T=T can be detected in lifeguards working on a beach in southern Sweden and that some kind of steady-state level is reached after longer exposures.
For the work on chemical exposures within the EU projects ECNIS and NewGeneris we have participated in the analysis of DNA adducts of acetaldehyde in people drinking alcohol and in a large human study in which it was showed that high levels of hemoglobin adducts of acrylamide in fetal blood was associated with a reduced birth weight.