Lecture: Improving high-resolution optical imaging for (neuro-)biology
Welcome to listen to Prof. Johann Georg Danzl, Institute of Science and Technology (IST) Austria, Klosterneuburg, Austria
Far-field optical super-resolution microscopy or nanoscopy techniques “super-resolve” features residing closer than the diffraction-limit by transiently preparing fluorophores in distinguishable (typically on- and off-) states and reading them out sequentially. In coordinate-targeted super-resolution modalities, such as stimulated emission depletion (STED) microscopy, this state difference is created by patterns of light, driving for instance all molecules to the off-state except for those residing at intensity minima.
I will discuss our recent efforts to improve coordinate-targeted nanoscopy. As a specific example, I will highlight how the use of multiple off-state transitions for nanoscopy can improve repeated imaging capability and on/off state contrast, enhancing image resolution and signal-to-background ratio in an approach that we dubbed “protected STED” (Danzl, Sidenstein et al., Nature Photonics (2016)). This allowed e.g. decoding the elaborate 3D structure of dendritic spines in living brain tissue.
I will also present the activities of my recently founded group at IST Austria where we work as an interdisciplinary team of physicists, biologists, and neuroscientists to develop and apply methods that bridge spatial scales from the nanoscale molecular arrangements to the native tissue context and where we strive to characterize biological samples with high information content.
Danzl JG, Sidenstein SC, Gregor C, Urban NT, Ilgen P, Jakobs S, Hell SW., Coordinate-targeted fluorescence nanoscopy with multiple off states, Nature Photonics 2016 10(2) 122-128
Fehrentz T, Huber FME, Hartrampf N, Bruegmann T, Frank JA, Fine NHF, Malan D, Danzl JG, Tikhonov DB, Sumser M, Sasse P, Hodson DJ, Zhorov BS, Klöcker N, Trauner D., Optical control of L-type Ca2+ channels using a diltiazem photoswitch. Nature Chemical Biology 2018 14(8):764-767.
Vladana VukojevicContact person: Vladana Vukojevic