Imaging at the atomic level
The Center has invested a significant amount of economic resources to amass cutting edge technology and scientific expertise.
Atomic visualization of the three-dimensional structures of proteins is essential for a complete understanding of the molecular basis underlying immune cell functions and development of infectious diseases. For this purpose, the Center has all required expertise in structural biology (X-ray crystallography) and biochemistry (production and isolation of soluble proteins) as well as several important techniques including circular dichroism and surface plasmon resonance.
Besides the state of the art crystallography facilities within the Center, we also have full access to X-ray robotic facilities in the Structural Genomic Consortium (SGC) at Karolinska Institutet. Sampling of diffraction data from protein crystals at the highest achievable resolution is performed on a routine basis in several European synchrotrons.
The ambition is to further develop the technical capacities of the Center in structural biology and biochemistry through the acquisition of additional state-of-the-art equipment, including novel Äkta Purifier with a specific refrigerator as well as temperature-controlled bacteria shaker, that allows for high performance purification, isolation and characterization of proteins.
We are planning to purchase additional instruments for dynamic light scattering, circular dichroism spectroscopy as well as a Surface Plasmon Resonance Biacore 3000 system that will allow for analyzes of protein binding affinity and thermodynamics.