Mouse Tissue Analysis
The Unit for Mouse Tissue Analysis (MTA) offer an automated immunohistochemistry service on mouse tissue. The advantages of using automated staining over manual staining include standardization and reproducibility between runs, and slides within a run as well as shorter running time for several applications.
A run can include up to 20 slides, and each slide in the run can be stained according to individual protocols, with varying pre-treatment, length and temperature of antibody incubation etc. We offer a panel of antibodies specific for different cell types in order to recognize these different cell types in tissues and help to classify tumour types (please see below). We also offer staining with other antibodies upon request.
We prefer tissues that have been fixed in 4% paraformaldehyde (PFA) for a preferred maximum time of 24 h. To ensure optimal fixation the volume of the fixative should be at least ten times the volume of the tissue. As an option, fixation could be done on a rocking platform to optimize penetration. Cut sections of 4 µm onto SuperFrostPlus slides, and air dry the slides in a vertical position over night. Baking of slides at higher temperatures is optional. If MTA needs to prepare sections, an extra cost will be charged.
In order to save reagents and time, multiple samples can be placed on the same slide. The automatic staining machine provides equal staining / hybridization conditions over the whole slide. Please contact MTA via e-mail before you start.
Projects involving new, previously untested antibodies will be judged on an individual basis and will be accepted only after thorough discussion with the customer.
Panel of antibodies:
Alpha-smooth muscle actin, F4/80, Vimentin, Nestin, Desmin, GFAP, Cytokeratins, Neurofilament, Ki67, cleaved caspase-3, PDGFR-alfa, PDGFR-beta.
This is a non-profit unit, but we do need to charge for materials and personnel time.
- Customer-provided primary antibody 200 SEK / slide
- MTA-provided primary antibody cheap 225 SEK / slide
- MTA-provided primary antibody expensive 300 SEK / slide
Immunohistochemistry (Other academic institutions)
- Customer-provided primary antibody 240 SEK / slide
- MTA-provided primary antibody cheap 270 SEK / slide
- MTA-provided primary antibody expensive 360 SEK / slide