StratCan workshop on CRISPR/Cas9
Theory & hands on workshop to design and use the CRISPR/Cas9 system for in vitro (cells) and in vivo (mice) applications.
The workshop takes place January 7-8, 2015 at KI Science Park, room Air & Fire (address: Tomtebodavägen 23 A).
- Kosuke Yusa, Wellcome Trust Sanger Institute
- Vera Grinkevich, Wellcome Trust Sanger Institute
- Feng Zhang, Broad Institute of MIT
- Haoyi Wang, 1. Chinese Academy of Sciences (CAS) 2. The Jackson Laboratory
The workshop is composed of:
A. Theory / Background
B. In vitro use (cells)
C. In vivo use (mice)
The final program will be tailor made according to the participants needs.
WEDNESDAY JAN 7
09.00 – 09.30 Galina Selivanova, Maria Kasper
Welcome & workshop introduction
09.30 – 10.30 Feng Zhang
Introduction to Genome Editing Using CRISPR-Cas9
10.30 – 11.00 COFFEE
11.00 – 12.00 Kosuke Yusa
Genome-wide loss-of-function screening using the CRISPR-Cas9 system
12.00 – 13.00 LUNCH
13.00 – 14.00 Vera Grinkevich
14.00 – 16.00 Kosuke Yusa & Vera Grinkevich
Supervising hands on training of participants to use web-based tools
16.00 – 18.00 Feng Zhang & Haoyi Wang
Discussion group: Advances and challenges of Crispr/Cas9
18.30 Dinner at Faculty club Svarta Rävfen, Aula Medica
THURSDAY JAN 8
09.00 – 10.00 Haoyi Wang
CRISPR-mediated genome engineering in mice
10.30 – 11.30 Feng Zhang
Advanced Application of CRISPR-Cas9 for Genome Perturbation
11.30 – 12.30 LUNCH
12.30 – 15.00 Haoyi Wang & Feng Zhang
In-depth discussion and hands on design to generate mouse models
Ex. Crisp/cas9 system to create point mutation, Cre/flox and reporter mice
Registration is closed (deadline December 1st) and all applicants will be notified soon (during week 50) regarding selection to the workshop that generated great interest (over 70 applicants).
The number of participants is limited to 30 (priority will be given to KI applicants) and a 600 SEK registration fee will apply (payment details will be provided with the acceptance notice). Please note that attendance during the full 2-day workshop is required.