Shared Laboratory equipment at FyFa

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A list of shared laboratory equipment available for use by all research groups at FyFa. The list also includes contact details to those responsible for each piece of equipment.

Rules for using the equipment

  1. Book the equipment in a calender (if available) next to the equipment.
  2. Read the instructions/manual and make sure you know how to use the equipment.
  3. If any problem occurs, please contact the person responsible for the equipment so that any required repairs can be made as soon as possible.
  4. All non-listed equipment belong to individual research groups and may only be used upon approval of the owners.

NanoDrop from Thermo Scientific

Manual is available next to the equipment.

The NanoDrop ND-1000 is a full-spectrom (220-750 nm) spectrophotometer that measures 1 ul samples with high accurnanodropacy and responsibility. It utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the ND-1000 has the capability to measure highly concentrated samples without dilution (50x higher concentration than the samples measured by a standard cuvette spectrophotometer).

Pharmacology: room 5325, corridor Schulte/Chagin

Contact:

Biomedical scientist

Åsa Nordling

Phone: +46-(0)8-524 877 60
Organizational unit: Ingelman-Sundberg Magnus group - Pharmacogenetics
E-mail: Asa.Nordling@ki.se

Physiology: room S95:30, corridor Zierath/Krook

Contact:

Laboratory manager

Katrin Ingermo

Phone: +46-(0)8-524 822 62
Organizational unit: Krook Anna group - Integrative Physiology
E-mail: Katrin.Ingermo@ki.se

Refrigerated Centrifuge Beckman GS-6Rrefrigerated-centrifugue-beckman-gs-6r

Max speed 3700 rpm. To be used with most vials from 1,5 ml to 50 ml. Large capacity

Pharmacology: room I 1310

Contact:

 

 

Biomedical scientist

Annika Olsson

Phone: +46-(0)8-524 879 71
Organizational unit: Lundberg Jon group - Pharmacological Nitric Oxide Research
E-mail: Annika.Olsson@ki.se

Cell and tissue disruptercell-and-tissue-disrupter

Sonicator Ultrasonic Processor W-385 Heat Systems

Pharmacology: room 5137, basement

Contact:

 

 

Biomedical scientist

Annika Olsson

Phone: +46-(0)8-524 879 71
Organizational unit: Lundberg Jon group - Pharmacological Nitric Oxide Research
E-mail: Annika.Olsson@ki.se

7500 FAST Real Time PCR from Applied Biosystems

Calendar booking and manual next to the machine.7500-fast-real-time-pcr-from-applied-biosystems

The Applied Biosystems 7500/7500 Fast Real-Time PCR System is a 96-well, five-color platform that uses fluorescence-based polymerase chain reaction (PCR) reagents to provide:
• Quantitative detection of target nucleic acid sequences (targets) using real-time analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs post-PCR).

Pharmacology: room 5325, corridor Shulte/Chagin

Contact:

Biomedical scientist

Åsa Nordling

Phone: +46-(0)8-524 877 60
Organizational unit: Ingelman-Sundberg Magnus group - Pharmacogenetics
E-mail: Asa.Nordling@ki.se

Step one plus Real Time PCR from Applied Biosystems

Calendar booking next to the machine.step-one-plus-real-time-pcr

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Physiology: room 219B, corridor Canlon/Westerblad

Contact:

TBC

CFX384 Real-Time PCR Detection System

Calendar booking next to the machine.cfx384-real-time-pcr-detection-system

Physiology: room 420, corridor Zierath/Krook.

Contact:

TBC

LSM T-PMT Confocal microscope from Zeiss

Single Molecules in the (Laser) Focus. Versatile measurements in solutions and cells: concentrations, interactions, fluctuationLSM-T-PMT-confocal-microscope-from-zeisss, diffusions - that's the ConfoCor 3. The ConfoCor 3 is directly attached to the channel output of the LSM 710. This choice of attachment gives you the highest flexibility for your required setup.

The combination provides 36 channels:
2x PMT, 1x Spectral detector with 32 channels and 2x APD

Imaris software

Pharmacology: room 5312, corridor Shulte/Chagin

Contact:

TBC

Fear conditioning (Med Associates Inc)

Fear conditioning is an established and commonly used system for studying contextual and cued fear learning, retention anfear-conditioning-med-associates-incd extinction in rodents. In classical fear conditioning, the animal associates a given cue (tone or light stimulus) with an electric shock. During a subsequent retention trial the animal is tested for its natural fear response (freezing) to the cue and/or the context. The fear conditioning software allows the free definition of trace & delay fear conditioning experimental designs and, using near infrared based imaging system it eliminates the need for visible light without influencing animal behavior. Fear conditioning is a good model for testing emotional learning and memory, and also anxiety-related disorders (hippocampal and amygdala-dependent memory). The apparatus is optimized for mice.

Pharmacology: behavioral corridor, basement

Contact:

TBC

Odyssey Imaging System from LI-CORodyssey-imaging-system-for-li-cor

Physiology: room 219B, corridor Canlon/Westerblad

Contact:

 

 

 

PhD student

Nilesh Agalave

Organizational unit: Svensson Camilla group - Molecular Pain Research
E-mail: nilesh.agalave@ki.se

Cryostat CM3050 S from LEICA

Calendar booking and manual available by the machine.cryostat-cm-3050-from-leica300

The Leica CM3050 S cryostat features electronic specimen feed (a more precise feed system than mechanical specimen feed) which gives the user a great advantage when absolute precision is a high priority. The unit also features motorized sectioning, encapsulated microtome power saving refrigeration system, programmable defrost cycles, twin-compressor cooling systems, a chamber temperature down to -40°C, specimen temperature down to -50°C, and a programmable reverse section counter. Section thickness can range from 0.5 to 300 microns, and maximum specimen size is 55 x 70 mm.

Pharmacology: room 5311, corridor Shulte/Chagin

Contact:

Senior researcher

Andrei Chagin

Phone: +46-(0)8-524 879 41
Organizational unit: Chagin Andrei group - Bone and Cartilage Physiology
E-mail: andrei.chagin@ki.se

Micro Beta 2460 LumiJet from Perkin Elmer

Calendar booking and manual available by the machine.micro-beta-2460-lumijet-from-perkin-elmer

Choice of single or dual channel injectors - with dual-channel injectors you can run dual aequorin (agonist and antagonist) screens without need of an external dispensing device. Available in 2 detector configurations. Multiple detectors can be used simultaneously to maximize throughput. For measuring luminescence and radioactivity(betacounter). Supports up to 384-well plates. Optional AssayPro data analysis software. AssayPro is a powerful, graphical presentation and analysis software tool that allows users to view and analyze data and identify "hits".

Pharmacology: room 5314, corridor Shulte/Chagin

Contact:

Research engineer

Eva Lindgren

Phone: +46-(0)8-524 875 85
Organizational unit: Education
E-mail: Eva.Lindgren@ki.se

Avanti J-26XP High speed centrifuge from Beckman Coulter

Calendar booking and manual available by the machine.avanti-j-26xp-high-speed-centrifugue-from-beckman-coulter

A fast, efficient centrifugation solution uniquely designed to process up to 6 liters in less than 10 minutes. Teamed with a full range of rotors, adaptors and sample carriers, this system delivers the versatility you need and the efficiency you deserve. Reduce hands-on sample handling with HarvestLine System Liners; offering convenient, easy to use improvements in biosafety and prolonged storage.

You have to buy your own tubes, make sure that they can handle the force you need to use in the centrifuge.

Pharmacology: room 5418, corridor Lundberg

Contact:

Biomedical scientist

Annika Olsson

Phone: +46-(0)8-524 879 71
Organizational unit: Lundberg Jon group - Pharmacological Nitric Oxide Research
E-mail: Annika.Olsson@ki.se

Biomedical scientist

Carina Nihlen

Phone: +46-(0)8-524 879 58
Organizational unit: Lundberg Jon group - Pharmacological Nitric Oxide Research
E-mail: Carina.Nihlen@ki.se

Sigma 2-5 centrifuge for microtiter plates

Calendar booking available by the machine.sigma-2-5-centrifugue-for-microtiter-plates

  • Laboratory centrifuge for 2 x 96 well microplate.
  • Simple 2-knob preselection of speed/RCF and time.
  • Short operation with display in seconds cycle.
  • Electronic regulated times of up to 30 minutes or continual run can be selected.
  • Efficient electrical brake for short deceleration and softstop for sensitive sedimentations.
  • As a matter of course this centrifuge includes a double cover closing and locking mechanism according to the German accident prevention regulation (VBG 7z). A LED-indicator signalizes when the rotor has stopped and the lid may be opened.
  • Indication of speed/RCF and time.
  • Product complient with national and international safety regulations (IEC 1010, CE).

Swing-out rotor for microtiter plates 11121

Swing-out rotor for microtiter plates, incl. 2 carriers no. 13223,
4000 rpm
2129 x g,
max. height of plates 50 mm

Pharmacology: room 5325, corridor Shulte/Chagin

Contact:

Biomedical scientist

Åsa Nordling

Phone: +46-(0)8-524 877 60
Organizational unit: Ingelman-Sundberg Magnus group - Pharmacogenetics
E-mail: Asa.Nordling@ki.se

Dry ice

Calendar booking and manual available by the machine.dry-ice

Dry ice has a very low temperature, -79 ° C. Handling is therefore appropriate protective gloves or other protective equipment used. The low temperature allows the freezing to occur rapidly.

Pharmacology: room, 5129, basement

Contact:

 

Technician

Michael Elm

Phone: +46-(0)70-791 25 72
Organizational unit: Administration
E-mail: Michael.Elm@ki.se

Please contact Michael Elm (Micke) when the gas is out/empty.

Evacuation freezer, -80°C.

When you use the freezers, put a note on the freezer stating your:evacuation-freezer-80-c

  • Name
  • Group
  • Phone number

Pharmacology: room 5150, basement

Contact:

 

Research engineer

Eva Lindgren

Phone: +46-(0)8-524 875 85
Organizational unit: Education
E-mail: Eva.Lindgren@ki.se

Physiology: room 03-035 ”Freezer 13”, basement

Contact:

Senior researcher

Camilla Svensson

Phone: +46-(0)8-524 879 48
Organizational unit: Svensson Camilla group - Molecular Pain Research
E-mail: camilla.svensson@ki.se

Beckman Optima MAX-XP Ultracentrifuge

The Optima MAX-XP Ultracentrifuge is a power-packed solution that delivers fast, efficient separations from samples as small as 175 μl up to 32.4 ml, and at speeds up to 150,000 RPM and more than 1,000,000 x g.beckman-optima-max-xp-ultracentrifugue

Rotor MLA-50: Tubes: Polyallomer (tubes needs to be full when running centrifuge. Can only be used once)
Max volume: 32.4ml Max RCF (xg): 233 000

Rotor MLA-80: Tubes: Thickwall polycarbonate. Re-usable, wash tubes after the run and put back in the drawer.
Max volume: 6.5 ml Max RCF (xg) : 417 000

Rotor TLA-100: Tubes: ThickwallPolyallomer
Max volume: 0,2 ml Max RCF (xg) 279 000

Physiology: level 2 room 237A corridor Gustafsson/Canlon/Westerblad

Contact:

Assistant professor

Johanna Lanner

Phone: +46-(0)8-524 823 73
Organizational unit: Lanner Johanna group - Molecular muscle physiology and pathophysiology
E-mail: Johanna.Lanner@ki.se

Research assistant

Niklas Ivarsson

Organizational unit: Westerblad Håkan group - Muscle Physiology
E-mail: niklas.ivarsson@ki.se

Radiocative Lab

You have to have a license and training to be allowed to work with isotopes at FyFa.

You need a login to get access.

Pharmacology: room 5143, basement

Contact:

Senior researcher

Souren Mkrtchian

Phone: +46-(0)8-524 877 92
Organizational unit: Ingelman-Sundberg Magnus group - Pharmacogenetics
E-mail: Souren.Mkrtchian@ki.se

Histo-core for paraffin sections

An in-house histology facility for paraffin embedding.

This includes a tissue-processing machine (for tissue dehydration), an embedding machine (for paraffin embedding) and microtome for sectioning of paraffin blocks.

Running costs: 5 kr/sample for the FyFa department, and 10 kr/sample for everybody else.

The capacity of the facility is quite high and we encourage you not only bringing your samples but also to advertise this service to all your colleagues. Intensive use will decrease the running cost.

Pharmacology: room 5141, basement

Contact:

Senior researcher

Andrei Chagin

Phone: +46-(0)8-524 879 41
Organizational unit: Chagin Andrei group - Bone and Cartilage Physiology
E-mail: andrei.chagin@ki.se

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