The MTC FACS Facility

The FACS (Fluorescence Activated Cell Sorter) Facility at MTC is intended for MTC and KI users. Other external users are also welcome if time is available. We offer high speed cell sorting as well as advanced and general flow cytometry analysis.

Instruments and Analysis

Instruments for Cell sorting

  •  MoFlo XDP with five lasers: Red 640nm, yellow/green 561nm, blue 488nm, violet 405nm, UV 355nm and 14 color detection and operated via Summit software. Four different cell populations can be sorted into collection tubes or multi-well plates (multiple formats) or slides with a refrigeration/heater option.
  •  BD FACSAria™ Fusion operated via FACSDiva software. The sorter has 4 lasers: Red 640nm, Yellow-green 561nm, Blue 488nm and Violet 405nm and can detect 16 fluorescence parameters. Four different cell populations can be sorted into collection tubes or multi-well plates (multiple formats) or slides with a refrigeration/heater option. This instrument can be used for BSL2 enhanced sorts.
  •  BD FACSJazz™ sorter operated via FACS Sortware. The sorter has 3 lasers: Red 640nm, Yellow-green 561nm and Blue 488nm and 6 fluorescence parameters. Two different cell populations can be sorted into collection tubes or multi-well plates with a refrigeration/heater option. This sorter is preferentially used for infected/transduced material, BSL2 enhanced.
  • FACSVantage/DiVa operated via FACSDiva software. The sorter has two laser units, one Dual, 488nm and UV 360nm, and one Multiline, 457.9-947.1nm, and five detectors for fluorescence. Four different cell populations can be sorted into collection tubes or multi-well plates (multiple formats) or slides with a refrigeration/heater option.

Cell sorts are performed by Birgitta Wester, Kiran Sedimbi and Maria Johansson.

Instruments for Cell Analysis

  • FACScan, with a 488 nm laser and three colors, linked to Mac and operated via CellQuestPro software.
  • FACSCalibur, with two lasers: 488 nm and 633 nm, four colors, linked to Mac and operated via CellQuestPro software.
  • CyAn ADP with three lasers: 488 nm, 635nm and 405nm, nine colors, linked to PC and operated via Summit software.
  • BD LSR II with four lasers, 488, 532, 640 and 405 nm, 18 colors, linked to a PC and operated via FACSDiva software.
  • FACSVerse with 3 lasers 488nm, 640nm and 405nm, 8 colors linked to a PC and operated via FACSSuite software. Equipped with a plate loader for sampling from 96-well plates and tube racks.

Computers for Data Analysis

  • A Mac G4 equipped with FlowJo, CellQuestPro software.
  • A PC workstation equipped with FACSSuite software and CBA (Cytometric Bead Array)

Contact

Facility Manager

Senior research specialist

Saikiran Sedimbi

Organizational unit: David Lane group
E-mail: Saikiran.Sedimbi@ki.se

Responsible for operations

Biomedical scientist

Birgitta Wester

Phone: +46-(0)8-524 867 39
Organizational unit: Pontus Aspenström group
E-mail: Birgitta.Wester@ki.se

Application Specialist

Senior researcher

Maria Johansson

Phone: +46-(0)8-524 863 70
Organizational unit: Klas Kärre group
E-mail: Maria.Johansson@ki.se

Links

Core facility