Karolinska Institutet Core Facilities
Karolinska Institutet Core Facilities is an umbrella definition for service laboratories, resources and competence centres whose activities are coordinated, supervised and partly financed by the Board of Research (FS) in collaboration with the local committees for Research and Education (FoUU) of the associated hospitals. Some of our core facilities are also managed and funded directly by departments and centers at KI.
Proteomics and Genomics
|The Advanced Mass Spectrometry Proteomics facility provide services using the best available proteomics techniques and methods not offered by any other facility in Sweden. Regional facility of national interest within SciLifeLab.|
|Bioinformatics and Expression Analysis (BEA)||Genomic analysis based on the Affymetrix, Agilent, Illumina and ABI platforms for microarrays, sequencing and qPCR. Regional facility of national interest within SciLifeLab.|
Assay development and screening, organic chemistry synthesis and analysis, compound handling and hosting of SciLifeLab compound collection (about 200 000 compounds). A national facility within SciLifeLab.
|Mass spectrometry based analysis to generate extensive data on protein level changes in biological samples. Regional facilities of national interest within SciLifeLab.|
|KI Gene - Genetic Analysis||Sanger DNA sequencing with fast turn-around time, gene expression analysis and analysis of genetic variations including SNPs, microsatellite markers and insertion/deletion polymorphisms.|
|Mutation Analysis Facility (MAF)||Targeted DNA methylation, genome-wide DNA methylation, genome-wide genotyping, targeted SNP genotyping as well as genome-wide expression analysis. Regional facilities of national interest within SciLifeLab.|
|Platforms for protein production, protein crystallography, and biophysical characterisation. National facility within SciLifeLab.|
|Proteomics Karolinska (PK/KI)||PK/KI provides services using best available proteomics techniques and methods with focus on label-free quantitative proteomics.|
This is a close collaboration between two cell imaging core-facilities at KI Campus Solna (north) – the CLICK facility and the CMB microscope facility. These core facilities are equipped with top-notch imaging systems suitable for live cell time-lapse, deep tissue, ion, spectral, confocal or general fluorescence imaging, and much more.
|Electron Tomography||Electron tomography permits subcellular and macromolecular assemblies to be studied in three dimensions.|
|Electron Microscopy Unit (EMil)|| |
Conventional preparation for TEM and SEM, preparation for immunohistochemistry, morphometric analysis, low temperature techniques such as low temperature embedding, high pressure freezing and cryosectioning (Tokuyasu method).
|Live Cell Imaging Core Facility (LCI)|| |
Advanced light microscopy facility with top-of-the-line widefield, confocal and light sheet microscopes. At the LCI facility you can laser-capture bits of tissues, print protein micropatterns, you can choose to run ultra-fast imaging, spectral detection, multiphoton excitation, FLIM measurements, long term live cell imaging, high throughput pipelines... The LCI facility puts education of researchers at the core of its activities with extensive user training and in-depth yearly microscopy course.
|MR Research Center||Handles the MR research capacity at the Neuroradiology Clinic.|
|A small animal imaging hub at Fleminsberg campus, and a part of the Preclinical Laboratory (PKL). PIF together with PKL provides a full range of preclinical study services for academia and industry sectors, providing them with high quality preclinical study data.|
|The Brain Bank||Clinical and experimental neuropathology. Manages collection of postmortem human brain and spinal cord tissue.|
|Karolinska Healthcare Research Biobank||Clinically evaluated fresh-frozen tumor tissues, including specimens from all major organs such as breast, prostate, endocrine organs, colon, brain, lung, etc.|
|KI Biobank||KI Biobank is aiming to support researchers who conduct research studies with sample collections, both within and outside Karolinska Institutet. The Biobank can assist with the collection, handling, storage and traceability of research samples.|
Biosafety and FACS
|Biosafety Level 3||The BSL-3 laboratory is platform to develop research work within the HIV field. It has been built for work with microorganisms classified BSL-3, with potential possibility of conversion to a BSL-4 unit.|
|MedH Flow Cytometry Facility||The facility offers a broad range of high quality services including assistance with experimental design, training for independent users, and operator-assisted services for cell analysis and cell sorting.|
|The MTC FACS Facility||High speed cell sorting as well as advanced and general flow cytometry analysis.|
|The CMB FACS Facility||High speed/purity cell sorting and advanced flow cytometry analysis.|
|VirusTech Core Facility||Genetically engineered viruses provide powerful tools for introducing desired genes in cells. To facilitate the use of this state-of-art methodology, the VirusTech facility at KI's Department of Cell and Molecular Biology specializes in the production, purification and concentration of such virus particles.|
Animal experimental facilities
|Karolinska Institutet Mouse Models (KIMM)||A core facility umbrella offering production, phenotype analyses, archiving of mouse mutants and experimental platforms to understand the role of genes and the microbiota. The KIMM umbrella facility includes the KCTT facility, the Germ-free facility, the Mouse Clinic, and an import portal (links in the list bellow).|
|Karolinska Center for Transgene Technologies (KCTT)||Generation and storage of genetically modified mice and related techniques. Part of Karolinska Institutet Mouse Models (KIMM).|
|Germ-free Research||The core facility provides germ-free models to academic researchers as well as to the biotech and pharmaceutical industries. Part of Karolinska Institutet Mouse Models (KIMM).|
|Mouse Clinic (KIMC)||KIMM's Mouse Clinic offers researchers the possibility to document the health of their genetically modified mouse strains according to international standards. Researchers may also chose between select analyses or a combination of analyses such as hematological-, physiological- and pathological and/or behavioural analyses.|
|KIMM Import Portal||The Import Portal (Portalen in Swedish) is the animal quarantine core facility at Karolinska Institutet Mouse Models.|
|Karolinska Experimental Research and Imaging Centre (KERIC)||Imaging analyses in experimental systems in combination with experimental surgery in large animals like swine as well as in rodents. This facility is managed by the Karolinska University Hospital in Solna (north campus area).|
|Preclinical Laboratory (PKL) Portal||The Preclinical Laboratory offers cryopreservation techniques and re-derivation services. Managed by the Karolinska University Hospital in Huddinge (south campus area).|
|Provides researchers with fish from wildtype lines, as well as offer the possibility of bringing in mutant/transgenic fish lines of interest from other laboratories.|
|Morphological Phenotype Analysis (FENO)||Specializing in the pathology of genetically engineered mice. Managed by the Karolinska University Hospital in Huddinge (south campus area).|
Other special infrastructures
|iPS Core – human iPS Cell Facility|| |
The reprogramming facility provides a reprogramming service to supply scientists with human induced pluripotent stem (iPS) cells. Patient-specific iPS cell lines opens up unique opportunities to in vitro study and create cellular models of human disease. The facility reprograms human fibroblast cells using Sendai viruses, which is a non-integrating method.
This facility is located at the Centre for Molecular Medicine and has the aim to facilitate the generation and distribution of human organoids from pluripotent stem cells, and to develop in vitro cellular models for academy and industry. The facility also provides support for the maintenance and differentiation of established human pluripotent stem cell lines – both human embryonic stem cells and human induced pluripotent stem cells – and training for researchers.
|Biostatistics core facility||The core facility provides biostatistical collaboration and support to medical and public health research to ensure that experimental designs, data analyses, and interpretation and dissemination of research findings take advantage of the most efficient and innovative methods in biostatistics.|
|Clinical Epidemiology||The core facility provides methodological support for researchers in clinical matters, both in planning and carrying out studies.|
|Karolinska Trial Alliance (KTA)||KTA is a clinical trials unit at Karolinska University Hospital in Stockholm, supported by the Stockholm County Council (SCC) and the Karolinska Institute.|
|The Swedish Twin Regstry||The Swedish Twin registry is a unique database and (inter)national resource. We are receptive to collaboration with academic and industry-based researchers.|
|Karolinska Cell Therapy Center (KCC) and Vecura|| |
KCC and Vecura supports and facilitates for researchers, clinicians and industrial companies that aim to develop quality assured Advanced Therapy Medicinal Products (ATMPs) and cell therapy products for unmet clinical needs.
Clinical sampling and sample analysis provided by healthcare professionals. This facility is runned by the Stockholm County Council.
Access to the Core Facilities is open to all researchers at KI and other academic institutions and also, to a limited extent, to commercial enterprises. Service laboratories and resource centres operate on a fee per service basis while competence centres aim to disseminate specific technologies and know-how on a collaborative basis. Policies regarding services, access, fees and prioritization vary by facility.
Core Facilities are managed by departments or units within Karolinska Institutet or associated hospitals.