DNA extraction at KI Biobank

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DNA extracted at KI Biobank has been utilized for genotyping and NGS applications with great success. KI Biobank offers the following services on DNA extraction and reformatting:

DNA extraction

Manual DNA extraction from 4-10 ml EDTA whole blood. The protocol is based on salt precipitation. DNA is dissolved in up to 1000 µl 10 mM Tris-HCl buffer, pH 8.0.

Automated DNA extraction. The protocol is based on magnetic bead separation. We offer DNA extraction from:

  • 400 µl whole blood from 4 ml EDTA tubes. Two whole blood backup tubes are created for future DNA extraction. DNA is dissolved in 150 µl 10 mM Tris-HCl buffer, pH 8.0.
  • 4 ml EDTA whole blood. DNA is dissolved in 500 µl 10 mM Tris-HCl buffer, pH 8.0.
  • 4 ml saliva (Oragene®-tubes). DNA is dissolved in 500 µl 10 mM Tris-HCl buffer, pH 8.0.

Concentration and purity of DNA is measured by UV-absorbance. Sample volume used in the measurement is 3 µl.

Reformatting

Dilution of DNA from stock solution to microtubes (DNA normalisation) and transfer to a PCR-plate. If wanted, we also transfer DNA to other tube formats.