Center for Live Imaging of Cells at Karolinska Institutet (CLICK)

The Center for Live Imaging of Cells at Karolinska Institutet (CLICK) is an advanced light microscopy facility equipped with confocal, 2-photon and fluorescence microscopes.

CLICK has the ambition to provide access to state-of-the-art microscopy equipment and all training and assistance required for basic observations of living cells to long-term time-lapse recordings of whole animals.

In addition, CLICK also provides access to software for data visualization, analysis and interpretation of 3D and 4D microscopy datasets.

Our equipment

Deep tissue imaging

  • Zeiss LSM510 confocal/two-photon with Meta detector for spectral imaging
  • Custom built Light Sheet (COLM) system

Confocal imaging

with motorized stage for tiling:

  • Zeiss LSM800
  • Zeiss LSM800 w Airy scan
  • Zeiss LSM700

with manual stage:

  • Olympus FV1000 CLSM

Calcium imaging

  • Zeiss upright widefield
  • Zeiss inverted widefield

Live Cell timelapse (temperature & CO2-control)

  • Zeiss Cell Observer
  • Chip-Man Technologies Cell-IQ
  • PerkinElmer Operetta HCS

Image processing & analysis workstations

  • Imaris
  • Amira
  • Image-Pro Plus 3D, version 7.0
  • AutoQuant X3-CWF
  • Zen 2010
  • Cell Analyzer
  • MATLAB

Regular widefield fluorescence

  • Zeiss Axioplan 2
  • Olympus IX73

Contact

If you are a researcher within KI and want to use our facility, you can request a PPMS account, and apply for a microscop training.

If you're not with KI you are welcome to e-mail us at bick@ki.se and we are happy to help you with your request!

Manager

Research engineer

Göran Månsson

Phone: +46-(0)8-524 879 77
Organizational unit: Per Uhléns group
E-mail: Goran.Mansson@ki.se

Director

Professor

Per Uhlén

Phone: +46-(0)8-524 876 77
Organizational unit: Per Uhléns group
E-mail: Per.Uhlen@ki.se

Find us

The CLICK Facility is situated in room A2-121 in the Retzius Laboratory at Karolinska Institutet.

The address is Division of Molecular NeuroBiology at MBB, Scheeles väg 1, 171 77 Stockholm, Sweden.

Prices

Pricelist CLICK

Rules of use

Rules of use CLICK

Links

Core facility