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Tuomas Näreoja

Postdoc

Visiting address : F56, Karolinska Universitetssjukhuset 14186 Stockholm, Sweden
Postal address : Department of Laboratory Medicine (LABMED), H5, Division of Pathology, F46, Karolinska Universitetssjukhuset, Huddinge 141 86 Stockholm, Sweden
Delivery address : F56 Via Varumottagningen, Karolinska Universitetssjukhuset 14186 Stockholm, Sweden

About me

Early 2017 I got funding to start assembling an independent research group working on regulation of bone remodeling and development of in vitro model to replicate the bone remodeling cycle. I did my PhD at University of Turku in Finland. Then I moved to Department Neurosciences at Karolinska institutet for a postdoc project on regulation synaptic vesicle membrane trafficking. After that I joined Division of Pathology in Department of Laboratory Medicine for another postdoc to study differentiation of osteoclasts.

Research description

We will develop an in vitro model recreating the entire bone remodeling cycle to examine in detail processes regulating bone remodeling and bone generation. My group’s long-term aim is to screen better small molecule drugs for osteoporosis and to produce bone for regenerative medicine. Bone remodeling is a constantly undergoing process where bone resorbing osteoclasts (OC), osteocytes (OY) inhabiting channels inside bone and bone mineralizing osteoblasts (OB) work together in order to heal damage and remake the skeleton, respond to mechanical stimulus and adapt bone structure to environmental factors and maintain Ca2+ and phosphate homeostasis. These cells receive regulatory signals through hormonal signaling network and transmit information to each other directly with cell-cell contacts, with soluble ligands as well as through surrounding extracellular matrix. I have developed an in vitro model of bone composed of sedimented bone nanoparticles facilitating easy high-resolution imaging to extract molecular detail in a relevant biological context. The primary aims of the proposed project are to:

1. further develop this model to include OYs, OCs, and OBs, and functionalization (e.g. proteins NO and pH-probes).

2. Elucidate and understand the coupling mechanisms in the intertwined signaling between OCs, OBs and OYs in mineralization. While OYs stimulate differentiation of OCs and OBs they also inhibit it by osteoprotegerin and sclerostin, respectively. In our model we can examine the impact of the signals in a sub-cellular resolution enabling us to establish precise molecular mechanisms.

3. Learn how to modulate balance between mineralization and resorption in an in vitro model replicating the bone-remodeling unit and to screen for drug candidates affecting the balance of the bone remodeling cycle.

 

Research team

Tuomas Näreoja

Laia Mira Pascual (PhD-student shared with G. Andersson and P. Lång)

Students wishing to do their M.Sc. thesis work in the group are welcome to contact tuomas.nareoja@ki.se

Academic honors, awards and prizes

Funding

Jane and Aatos Erkko foundation

Loo and Hans Osterman foundation

Maud Kuistila memorial foundation

Publications

Publications

Diversity of actin architecture in human osteoclasts: network of curved and branched actin supporting cell shape and intercellular micrometer-level tubes
Pennanen P, Alanne Mh, Fazeli E, Deguchi T, Näreoja T, Peltonen S, et al
Molecular and cellular biochemistry 2017;():-

Ratiometric Sensing and Imaging of Intracellular pH Using Polyethylenimine-Coated Photon Upconversion Nanoprobes
Näreoja T, Deguchi T, Christ S, Peltomaa R, Prabhakar N, Fazeli E, et al
Analytical chemistry 2017;89(3):1501-1508

Super-sensitive time-resolved fluoroimmunoassay for thyroid-stimulating hormone utilizing europium(III) nanoparticle labels achieved by protein corona stabilization, short binding time, and serum preprocessing
Näreoja T, Rosenholm Jm, Lamminmäki U, Hänninen Pe
Analytical and bioanalytical chemistry 2017;409(13):3407-3416

Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane
Wen Pj, Grenklo S, Arpino G, Tan X, Liao Hs, Heureaux J, et al
Nature communications 2016;7():12604-

Advanced Cellulose Fibers for Efficient Immobilization of Enzymes
Vega Erramuspe Ib, Fazeli E, Näreoja T, Trygg J, Hänninen P, Heinze T, et al
Biomacromolecules 2016;17(10):3188-3197

In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts
Deguchi T, Alanne Mh, Fazeli E, Fagerlund Km, Pennanen P, Lehenkari P, et al
Scientific reports 2016;6():22585-

Modulation of the structural properties of mesoporous silica nanoparticles to enhance the T-1-weighted MR imaging capability
Sen Karaman D, Desai D, Zhang Jx, Tadayon S, Unal G, Teuho J, et al
JOURNAL OF MATERIALS CHEMISTRY B 2016;4(9):1720-1732

Prolonged Dye Release from Mesoporous Silica-Based Imaging Probes Facilitates Long-Term Optical Tracking of Cell Populations In Vivo
Rosenholm Jm, Gulin-sarfraz T, Mamaeva V, Niemi R, Özliseli E, Desai D, et al
Small (Weinheim an der Bergstrasse, Germany) 2016;12(12):1578-92

Residual nanoparticle label immunosensor for wash-free C-reactive protein detection in blood
Huttunen Rj, Näreoja T, Mariani L, Härmä H
Biosensors & bioelectronics 2016;83():54-9

Stimuli-responsive hybrid nanocarriers developed by controllable integration of hyperbranched PEI with mesoporous silica nanoparticles for sustained intracellular siRNA delivery
Prabhakar N, Zhang Jx, Desai D, Casals E, Gulin-sarfraz T, Nareoja T, et al
INTERNATIONAL JOURNAL OF NANOMEDICINE 2016;:6591-6608

An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction
Winther Åm, Vorontsova O, Rees Ka, Näreoja T, Sopova E, Jiao W, et al
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015;35(44):14756-70

Functionalization of graphene oxide nanostructures improves photoluminescence and facilitates their use as optical probes in preclinical imaging
Prabhakar N, Nareoja T, Von Haartman E, Sen Karaman D, Burikov Sa, Dolenko Ta, et al
NANOSCALE 2015;7(23):10410-20

Nanometric features of myosin filaments extracted from a single muscle fiber to uncover the mechanisms underlying organized motility
Li M, Deguchi T, Näreoja T, Jena Bp, Hänninen P, Larsson L
Archives of biochemistry and biophysics 2015;583():1-8

Preparation of reactive fibre interfaces using multifunctional cellulose derivatives
Vega B, Wondraczek H, Bretschneider L, Nareoja T, Fardim P, Heinze T
CARBOHYDRATE POLYMERS 2015;:261-73

Axial Super-resolution by Mirror-reflected Stimulated Emission Depletion Microscopy
Deguchi T, Koho S, Nareoja T, Hanninen P
OPTICAL REVIEW 2014;21(3):389-394

Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay
Nareoja T, Ebner A, Gruber Hj, Taskinen B, Kienberger F, Hanninen Pe, et al
ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2014;406(2):493-503

p38 delta mitogen-activated protein kinase regulates the expression of tight junction protein ZO-1 in differentiating human epidermal keratinocytes
Siljamaki E, Raiko L, Toriseva M, Nissinen L, Nareoja T, Peltonen J, et al
ARCHIVES OF DERMATOLOGICAL RESEARCH 2014;306(2):131-41

Photon upconversion sensitized nanoprobes for sensing and imaging of pH
Arppe R, Nareoja T, Nylund S, Mattsson L, Koho S, Rosenholm Jm, et al
NANOSCALE 2014;6(12):6837-43

Semiconducting Polymer Encapsulated Mesoporous Silica Particles with Conjugated Europium Complexes: Toward Enhanced Luminescence under Aqueous Conditions
Zhang Jx, Prabhakar N, Nareoja T, Rosenholm Jm
ACS APPLIED MATERIALS & INTERFACES 2014;6(21):19064-74

Core-shell designs of photoluminescent nanodiamonds with porous silica coatings for bioimaging and drug delivery II: application
Prabhakar N, Nareoja T, Von Haartman E, Sen Karaman D, Jiang H, Koho S, et al
NANOSCALE 2013;5(9):3713-22

NATURAL PRODUCT DRUG DISCOVERY FOR CHLAMYDIA PNEUMONIAE
Salin O, Pohjala L, Nareoja T, Vuorela P
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 2011;:17-18

Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labels
Nareoja T, Maattanen A, Peltonen J, Hanninen Pe, Harma H
JOURNAL OF IMMUNOLOGICAL METHODS 2009;347(1-2):24-30

Study on nonspecificity of an immuoassay using Eu-doped polystyrene nanoparticle labels
Nareoja T, Vehniainen M, Lamminmaki U, Hanninen Pe, Harma H
JOURNAL OF IMMUNOLOGICAL METHODS 2009;345(1-2):80-9

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