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Margaret Sällberg Chen

Senior lecturer

Visiting address : Alfred Nobels Allé 8 141 52 Huddinge, Sweden
Postal address : Department of Dental Medicine (DENTMED), OF, Division 2, Cariology, Endodontics, Dental Biomaterials, Dental Technician Training, Box 4064 141 04 Huddinge, Sweden
Delivery address : Alfred Nobels Allé 8, Plan 7 141 52 Huddinge, Sweden

About me

Associate Professor and Senior Lecturer at Karolinska Institutet. Director of Doctoral Studies and Head of International Affairs at Department of Dental Medicine.

Margaret Sällberg Chen received her D.D.S. and Ph.D. degrees from Karolinska Institutet. Following postdoctoral training at The Scripps Research Institute where she developed T cell receptor transgene models for immunological studies of chronic viral infection, she was recruited to the former Swedish Institute for Infectious Disease Control to continue with immunology and vaccinology studies. In 2004 she was appointed associated professor at Karolinska Institutet, and senior lecturer at Department of Dental Medicine in 2009. She has co-authored more than 50 papers, and has served on national and international scientific and editorial board. 

Research description

The lab is broadly interested in immunological mechanisms effective in eliminating microbial pathogens associated with chronic diseases and tumors. The research pursues several lines investigating cellular functions capable of sensing and killing intracellular pathogens and tumors, and the target identification. The models are exclusively human infectious diseases and the methodologies employed include synthetic genetics, genetically modified cells and animals, viral and non-viral vectors, and next generation sequencing.

Major research area: Genetic transfer of T cell receptors, redirection of lymphocytes, viral and neo-antigens, therapeutic targets in oral conditions including tumors and endodontic infections.

 

Documents

Publications

Ameloblastoma RNA profiling uncovers a distinct non-coding RNA signature
Davanian H, Balasiddaiah A, Heymann R, Sundström M, Redenström P, Silfverberg M, et al
Oncotarget 2017;8(3):4530-4542

Hepatitis C Virus-Specific T Cell Receptor mRNA-Engineered Human T Cells: Impact of Antigen Specificity on Functional Properties
Balasiddaiah A, Davanian H, Aleman S, Pasetto A, Frelin L, Sällberg M, et al
Journal of virology 2017;91(9):-

Functional differences in hepatitis C virus nonstructural (NS) 3/4A- and 5A-specific T cell responses
Holmström F, Chen M, Balasiddaiah A, Sällberg M, Ahlén G, Frelin L
Scientific reports 2016;6():24991-

Prospects and progress of DNA vaccines for treating hepatitis B
Chen M, Jagya N, Bansal R, Frelin L, Sällberg M
Expert review of vaccines 2016;15(5):629-40

Electroporation for therapeutic DNA vaccination in patients
Sällberg M, Frelin L, Ahlén G, Sällberg-chen M
Medical microbiology and immunology 2015;204(1):131-5

A randomized controlled trial: the efficacy of eluoride rinse combined with calcium pre-rinse to increase overnight salivary fluoride
Chen Ms, Strömberg E, Vogel Gl, Sandborgh-englund G
Acta odontologica Scandinavica 2014;72(7):557-60

Functional attributes of responding T cells in HCV infection: the recent advances in engineering functional antiviral T cells
Pasetto A, Aleman S, Chen M
Archivum immunologiae et therapiae experimentalis 2014;62(1):23-30

A synthetic codon-optimized hepatitis C virus nonstructural 5A DNA vaccine primes polyfunctional CD8+ T cell responses in wild-type and NS5A-transgenic mice
Holmström F, Pasetto A, Nähr V, Brass A, Kriegs M, Hildt E, et al
Journal of immunology (Baltimore, Md. : 1950) 2013;190(3):1113-24

Containing "The Great Houdini" of viruses: combining direct acting antivirals with the host immune response for the treatment of chronic hepatitis C
Ahlén G, Frelin L, Brenndörfer Ed, Brass A, Weiland O, Chen M, et al
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2013;16(3-5):60-7

Generation of T-cell receptors targeting a genetically stable and immunodominant cytotoxic T-lymphocyte epitope within hepatitis C virus non-structural protein 3
Pasetto A, Frelin L, Brass A, Yasmeen A, Koh S, Lohmann V, et al
JOURNAL OF GENERAL VIROLOGY 2012;:247-58

Signaling pathways involved in the regulation of TNF alpha-induced toll-like receptor 2 expression in human gingival fibroblasts
Davanian H, Bage T, Lindberg J, Lundeberg J, Concha Hq, Chen Ms, et al
CYTOKINE 2012;57(3):406-16

TCR-Redirected Human T Cells Inhibit Hepatitis C Virus Replication: Hepatotoxic Potential Is Linked to Antigen Specificity and Functional Avidity
Pasetto A, Frelin L, Aleman S, Holmstrom F, Brass A, Ahlen G, et al
JOURNAL OF IMMUNOLOGY 2012;189(9):4510-9

Heterologous T Cells Can Help Restore Function in Dysfunctional Hepatitis C Virus Nonstructural 3/4A-Specific T Cells during Therapeutic Vaccination
Chen A, Ahlen G, Brenndorfer Ed, Brass A, Holmstrom F, Chen M, et al
JOURNAL OF IMMUNOLOGY 2011;186(9):5107-18

Role of innate signalling pathways in the immunogenicity of alphaviral replicon-based vaccines
Naslund Ti, Kostic L, Nordstrom Ekl, Chen M, Liljestrom P
VIROLOGY JOURNAL 2011;:36-

ELECTROPORATION: A PROMISING METHOD FOR THE NONVIRAL DELIVERY OF DNA VACCINES IN HUMANS?
Frelin L, Brass A, Ahlen G, Brenndorfer Ed, Chen M, Sallberg M
DRUG NEWS & PERSPECTIVES 2010;23(10):647-53

Augmentation of alphavirus vector-induced human papilloma virus-specific immune and anti-tumour responses by co-expression of interleukin-12
Riezebos-brilman A, Regts J, Chen M, Wilschut J, Daemen T
VACCINE 2009;27(5):701-7

Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C
Gomez Ce, Najera Jl, Jimenez V, Bieler K, Wild J, Kostic L, et al
VACCINE 2007;25(11):1969-92

Immune tolerance split between hepatitis B virus precore and core proteins
Chen M, Sallberg M, Hughes J, Jones J, Guidotti Lg, Chisari Fv, et al
JOURNAL OF VIROLOGY 2005;79(5):3016-27

MyD88 expression is required for efficient cross-presentation of viral antigens from infected cells
Chen M, Barnfield C, Naslund Ti, Fleeton Mn, Liljestrom P
JOURNAL OF VIROLOGY 2005;79(5):2964-72

The immunomodulating properties of human respiratory syncytial virus and immunostimulating complexes containing Quillaja saponin components QH-A, QH-C and ISCOPREP (TM) 703
Hu Kf, Regner M, Siegrist Ca, Lambert P, Chen M, Bengtsson Kl, et al
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 2005;43(2):269-76

Toll-like receptor 3 promotes cross-priming to virus-infected cells
Schulz O, Diebold Ss, Chen M, Naslund Ti, Nolte Ma, Alexopoulou L, et al
NATURE 2005;433(7028):887-92

A function of the hepatitis B virus precore protein is to regulate the immune response to the core antigen
Chen Mt, Billaud Jn, Sallberg M, Guidotti Lg, Chisari Fv, Jones J, et al
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2004;101(41):14913-8

Hepatitis C virus non-structural protein 3-specific cellular immune responses following single or combined immunization with DNA or recombinant Semliki Forest virus particles
Brinster C, Chen M, Boucreux D, Paranhos-baccala G, Liljestrom P, Lemmonier F, et al
JOURNAL OF GENERAL VIROLOGY 2002;:369-81

Immunotherapy with recombinant SFV-replicons expressing the P815A tumor antigen or IL-12 induces tumor regression
Colmenero P, Chen M, Castanoz-velez E, Liljestrom P, Jondal M
INTERNATIONAL JOURNAL OF CANCER 2002;98(4):554-60

Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus
Chen M, Hu Kf, Rozell B, Orvell C, Morein B, Lijestrom P
JOURNAL OF IMMUNOLOGY 2002;169(6):3208-16

Different respiratory syncytial virus and Quillaja saponin formulations induce murine peritoneal cells to express different proinflammatory cytokine profiles
Hu Kf, Chen M, Abusugra I, Monaco F, Morein B
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 2001;31(2):105-12

Humoral and CD4(+) T helper (Th) cell responses to the hepatitis C virus non-structural 3 (NS3) protein: NS3 primes Th1-like responses more effectively as a DNA-based immunogen than as a recombinant protein
Lazdina U, Hultgren C, Frelin L, Chen M, Lodin K, Weiland O, et al
The Journal of general virology 2001;82(Pt 6):1299-308

Modeling the T-helper cell response in acute and chronic hepatitis B virus infection using T-cell receptor transgenic mice
Chen M, Sallberg M, Thung Sn, Hughes J, Jones J, Milich Dr
ANTIVIRAL RESEARCH 2001;52(2):99-111

Self-replicative RNA vaccines elicit protection against influenza A virus, respiratory syncytial virus, and a tickborne encephalitis virus
Fleeton Mn, Chen M, Berglund P, Rhodes G, Parker Se, Murphy M, et al
JOURNAL OF INFECTIOUS DISEASES 2001;183(9):1395-8

Changing profile of HIV-1 serotypes in Iceland during 1989-96
Love A, Chen M, Sallberg M
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES 2000;32(4):445-6

Characterization of a monoclonal antibody and its single-chain antibody fragment recognizing the nucleoside triphosphatase/helicase domain of the hepatitis C virus nonstructural 3 protein
Zhang Zx, Lazdina U, Chen M, Peterson Dl, Sallberg M
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 2000;7(1):58-63

Humoral and cellular immune responses to the GB virus C hepatitis G virus envelope 2 protein
Lazdina U, Hultgren C, Chen M, Fischler B, Weiland O, Mushahwar Ik, et al
JOURNAL OF MEDICAL VIROLOGY 2000;62(3):334-44

Nondeletional T-cell receptor transgenic mice: Model for the CD4(+) T-cell repertoire in chronic hepatitis B virus infection
Chen M, Sallberg M, Thung Sn, Hughes J, Jones J, Milich Dr
JOURNAL OF VIROLOGY 2000;74(16):7587-99

Use of conventional or replicating nucleic acid-based vaccines and recombinant Semliki Forest virus-derived particles for the induction of immune responses against hepatitis C virus core and E2 antigens
Vidalin O, Fournillier A, Renard N, Chen M, Depla E, Boucreux D, et al
VIROLOGY 2000;276(2):259-70

Analysis of GB virus C markers in families over three generations
Chen M, Fischler B, Hultgren C, Halasz R, Nemeth A, Sallberg M
JOURNAL OF CLINICAL MICROBIOLOGY 1999;37(12):4153-5

Limited humoral immunity in hepatitis C virus infection
Chen M, Sallberg M, Sonnerborg A, Weiland O, Mattsson L, Jin L, et al
GASTROENTEROLOGY 1999;116(1):135-43

Human and murine antibody recognition is focused on the ATPase/helicase, but not the protease domain of the hepatitis C virus nonstructural 3 protein
Chen M, Sallberg M, Sonnerborg A, Jin L, Birkett A, Peterson D, et al
HEPATOLOGY 1998;28(1):219-24

The secreted hepatitis B precore antigen can modulate the immune response to the nucleocapsid: A mechanism for persistence
Milich Dr, Chen Mk, Hughes Jl, Jones Je
JOURNAL OF IMMUNOLOGY 1998;160(4):2013-21

Characterization of humoral and CD4(+) cellular responses after genetic immunization with retroviral vectors expressing different forms of the hepatitis B virus core and e antigens
Sallberg M, Townsend K, Chen M, Odea J, Banks T, Jolly Dj, et al
JOURNAL OF VIROLOGY 1997;71(7):5295-303

Detection of hepatitis G virus (GB virus C) RNA in human saliva
Chen M, Sonnerborg A, Johansson B, Sallberg M
JOURNAL OF CLINICAL MICROBIOLOGY 1997;35(4):973-5

Genetic evidence for mother-to-infant transmission of hepatitis G virus
Fischler B, Lara C, Chen M, Sonnerborg A, Nemeth A, Sallberg M
JOURNAL OF INFECTIOUS DISEASES 1997;176(1):281-5

Immune responses to the hepatitis C virus NS4A protein are profoundly influenced by the combination of the viral genotype and the host major histocompatibility complex
Zhang Zx, Chen M, Hultgren C, Birkett A, Milich Dr, Sallberg M
JOURNAL OF GENERAL VIROLOGY 1997;:2735-46

Role of B cells in antigen presentation of the hepatitis B core
Milich Dr, Chen M, Schodel F, Peterson Dl, Jones Je, Hughes Jl
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1997;94(26):14648-53

Immunogenicity and antigenicity of the ATPase/helicase domain of the hepatitis C virus non-structural 3 protein
Sallberg M, Zhang Zx, Chen M, Jin L, Birkett A, Peterson Dl, et al
JOURNAL OF GENERAL VIROLOGY 1996;:2721-8

DETECTION OF HEPATITIS-C VIRUS-RNA IN THE CELL FRACTION OF SALIVA BEFORE AND AFTER ORAL-SURGERY
Chen M, Yun Zb, Sallberg M, Schvarcz R, Bergquist I, Berglund Hb, et al
JOURNAL OF MEDICAL VIROLOGY 1995;45(2):223-6

DISTINGUISHING ACUTE FROM SYMPTOMATIC CHRONIC HEPATITIS-C VIRUS (HCV) INFECTION BY SITE-DIRECTED SEROLOGY OF THE HCV STRUCTURAL PROTEINS
Zhang Zx, Chen M, Sonnerborg A, Weiland O, Sallberg M
JOURNAL OF INFECTIOUS DISEASES 1995;171(5):1356-9

Efficacy of human leucocyte alpha-interferon treatment for chronic hepatitis C virus infection
Weiland O, Chen M, Lindh G, Mattsson L, Schvarcz R, Sönnerborg A, et al
Scandinavian journal of infectious diseases 1995;27(4):319-24

Efficacy of human leucocyte alpha-interferon treatment for chronic hepatitis C virus infection
Weiland O, Chen M, Lindh G, Mattsson L, Schvarcz R, Sönnerborg A, et al
Scandinavian journal of infectious diseases 1995;27(5):319-24

EFFICACY OF HUMAN-LEUKOCYTE ALPHA-INTERFERON TREATMENT FOR CHRONIC HEPATITIS-C VIRUS-INFECTION
Weiland O, Chen M, Lindh G, Mattsson L, Schvarcz R, Sonnerborg A, et al
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES 1995;27(4):319-324

EVALUATION OF A MULTIPLE PEPTIDE ASSAY FOR TYPING OF ANTIBODIES TO THE HEPATITIS-C VIRUS - RELATION TO GENOMIC TYPING BY THE POLYMERASE CHAIN-REACTION
Zhang Zx, Yun Zb, Chen M, Sonnerborg A, Sallberg M
JOURNAL OF MEDICAL VIROLOGY 1995;45(1):50-5

LEVELS OF HEPATITIS-C VIRUS (HCV) RNA IN SERUM AND THEIR RELATIONSHIP TO LEVELS OF IMMUNOGLOBULIN-M AND IMMUNOGLOBULIN-G ANTIBODIES AGAINST HCV CORE PROTEIN
Chen M, Sonnerborg A, Sallberg M
JOURNAL OF CLINICAL MICROBIOLOGY 1995;33(3):778-80

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