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Elias Arnér

Professor

About me

Professor in biochemistry

Background:

MD PhD degrees from Karolinska Institutet, through the MD PhD program (LäFo), as one of the 10 students first admitted when the program was launched in 1986. 

Post doc, Ludwig Maximilian University, Munich, Germany, 1998-1999.

Docent in medical biochemistry, 2000.

Dean of postgraduate education at Karolinska Institutet, 2005-2007.

Head of the biochemistry division at MBB since 2008.

Professor in biochemistry with special focus on selenium research in 2009.

Executive Editor, BBA General Subjects, since 2008.

Editor, JBC, since 2011.

Faculty representative at the University Board (Konsistoriet) of Karolinska Institutet, since 2011.

Research description

We are studying the role and function of selenoproteins. A main focus is on the mammalian thioredoxin system, predominantly the expression, regulation and function of the selenoprotein thioredoxin reductase 1 (TrxR1) and its splice variants, especially in relation to cellular signaling events and in cancer.  

Another focus of research is the development of production systems for selenoproteins and their potential use in diverse biotechnological applications.

For more information on our research, see the links below.

Documents

Links

Publications

APR-246/PRIMA-1 inhibits thioredoxin reductase 1 and converts the enzyme to a dedicated NADPH oxidase
Peng X, Zhang Mq, Conserva F, Hosny G, Selivanova G, Bykov Vj, et al
Cell death & disease 2017;8(4):e2751-

Cross Talk in HEK293 Cells Between Nrf2, HIF, and NF-κB Activities upon Challenges with Redox Therapeutics Characterized with Single-Cell Resolution
Johansson K, Cebula M, Rengby O, Dreij K, Carlström Ke, Sigmundsson K, et al
Antioxidants & redox signaling 2017;26(6):229-246

Cytosolic thioredoxin reductase 1 is required for correct disulfide formation in the ER
Poet Gj, Oka Ob, Van Lith M, Cao Z, Robinson Pj, Pringle Ma, et al
The EMBO journal 2017;36(5):693-702

Hepatocyte Hyperproliferation upon Liver-Specific Co-disruption of Thioredoxin-1, Thioredoxin Reductase-1, and Glutathione Reductase
Prigge Jr, Coppo L, Martin Ss, Ogata F, Miller Cg, Bruschwein Md, et al
Cell reports 2017;19(13):2771-2781

Homozygous mutation in TXNRD1 is associated with genetic generalized epilepsy
Kudin Ap, Baron G, Zsurka G, Hampel Kg, Elger Ce, Grote A, et al
Free radical biology & medicine 2017;106():270-277

Rutin protects against HO-triggered impaired relaxation of placental arterioles and induces Nrf2-mediated adaptation in Human Umbilical Vein Endothelial Cells exposed to oxidative stress
Sthijns Mmjpe, Schiffers Pm, Janssen Gm, Lemmens Kja, Ides B, Vangrieken P, et al
Biochimica et biophysica acta 2017;1861(5 Pt A):1177-1189

Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation
Cheng Q, Arnér Es
The Journal of biological chemistry 2017;292(13):5476-5487

Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during HO exposure
Dagnell M, Pace Pe, Cheng Q, Frijhoff J, Östman A, Arnér Esj, et al
The Journal of biological chemistry 2017;():-

Time- and cell-resolved dynamics of redox-sensitive Nrf2, HIF and NF-κB activities in 3D spheroids enriched for cancer stem cells
Kipp Ap, Deubel S, Arnér Esj, Johansson K
Redox biology 2017;12():403-409

A novel persulfide detection method reveals protein persulfide- and polysulfide-reducing functions of thioredoxin and glutathione systems
Dóka É, Pader I, Bíró A, Johansson K, Cheng Q, Ballagó K, et al
Science advances 2016;2(1):e1500968-

Chemical Reactivity Window Determines Prodrug Efficiency toward Glutathione Transferase Overexpressing Cancer Cells
Van Gisbergen Mw, Cebula M, Zhang J, Ottosson-wadlund A, Dubois L, Lambin P, et al
Molecular pharmaceutics 2016;13(6):2010-25

Details in the catalytic mechanism of mammalian thioredoxin reductase 1 revealed using point mutations and juglone-coupled enzyme activities
Xu J, Cheng Q, Arnér Es
Free radical biology & medicine 2016;94():110-20

Entinostat up-regulates the CAMP gene encoding LL-37 via activation of STAT3 and HIF-1α transcription factors
Miraglia E, Nylén F, Johansson K, Arnér E, Cebula M, Farmand S, et al
Scientific reports 2016;6():33274-

Indolin-2-one compounds targeting thioredoxin reductase as potential anticancer drug leads
Kaminska Kk, Bertrand Hc, Tajima H, Stafford Wc, Cheng Q, Chen W, et al
ONCOTARGET 2016;7(26):40233-40251

Inhibitory nitrosylation of mammalian thioredoxin reductase 1: Molecular characterization and evidence for its functional role in cellular nitroso-redox imbalance
Engelman R, Ziv T, Arnér Es, Benhar M
Free radical biology & medicine 2016;97():375-85

Paradoxical Roles of Antioxidant Enzymes: Basic Mechanisms and Health Implications
Lei Xg, Zhu Jh, Cheng Wh, Bao Y, Ho Ys, Reddi Ar, et al
Physiological reviews 2016;96(1):307-64

Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake
Cheng Q, Wållberg H, Grafström J, Lu L, Thorell Jo, Hägg Olofsson M, et al
EJNMMI research 2016;6(1):58-

Selenoprotein Gene Nomenclature
Gladyshev Vn, Arnér Es, Berry Mj, Brigelius-flohé R, Bruford Ea, Burk Rf, et al
The Journal of biological chemistry 2016;291(46):24036-24040

Serum thioredoxin reductase is highly increased in mice with hepatocellular carcinoma and its activity is restrained by several mechanisms
Zhang L, Cheng Q, Zhang L, Wang Y, Merrill Gf, Ilani T, et al
Free radical biology & medicine 2016;99():426-435

Thioredoxin reductase 1 suppresses adipocyte differentiation and insulin responsiveness
Peng Xx, Gimenez-cassina A, Petrus P, Conrad M, Ryden M, Arner Esj
SCIENTIFIC REPORTS 2016;:28080-

Dietary methionine can sustain cytosolic redox homeostasis in the mouse liver
Eriksson S, Prigge Jr, Talago Ea, Arnér Es, Schmidt Ee
Nature communications 2015;6():6479-

Redox effects and cytotoxic profiles of MJ25 and auranofin towards malignant melanoma cells
Sachweh Mc, Stafford Wc, Drummond Cj, Mccarthy Ar, Higgins M, Campbell J, et al
Oncotarget 2015;6(18):16488-506

The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death
Xu J, Eriksson Se, Cebula M, Sandalova T, Hedström E, Pader I, et al
Cell death & disease 2015;6():e1616-

TrxR1 as a potent regulator of the Nrf2-Keap1 response system
Cebula M, Schmidt Ee, Arnér Es
Antioxidants & redox signaling 2015;23(10):823-53

Cisplatin and oxaliplatin are toxic to cochlear outer hair cells and both target thioredoxin reductase in organ of Corti cultures
Dammeyer P, Hellberg V, Wallin I, Laurell G, Shoshan M, Ehrsson H, et al
Acta oto-laryngologica 2014;134(5):448-54

Redox active motifs in selenoproteins
Li F, Lutz Pb, Pepelyayeva Y, Arnér Es, Bayse Ca, Rozovsky S
Proceedings of the National Academy of Sciences of the United States of America 2014;111(19):6976-81

ROS-dependent activation of JNK converts p53 into an efficient inhibitor of oncogenes leading to robust apoptosis
Shi Y, Nikulenkov F, Zawacka-pankau J, Li H, Gabdoulline R, Xu J, et al
Cell death and differentiation 2014;21(4):612-23

Sec-containing TrxR1 is essential for self-sufficiency of cells by control of glucose-derived H2O2
Peng X, Mandal Pk, Kaminskyy Vo, Lindqvist A, Conrad M, Arnér Es
Cell death & disease 2014;5():e1235-

Sepp1(UF) forms are N-terminal selenoprotein P truncations that have peroxidase activity when coupled with thioredoxin reductase-1
Kurokawa S, Eriksson S, Rose Kl, Wu S, Motley Ak, Hill S, et al
Free radical biology & medicine 2014;69():67-76

Serum thioredoxin reductase levels increase in response to chemically induced acute liver injury
Sun K, Eriksson Se, Tan Y, Zhang L, Arnér Es, Zhang J
Biochimica et biophysica acta 2014;1840(7):2105-11

The 19S Deubiquitinase inhibitor b-AP15 is enriched in cells and elicits rapid commitment to cell death
Wang X, Stafford W, Mazurkiewicz M, Fryknäs M, Brjnic S, Zhang X, et al
Molecular pharmacology 2014;85(6):932-45

Thioredoxin-related protein of 14 kDa is an efficient L-cystine reductase and S-denitrosylase
Pader I, Sengupta R, Cebula M, Xu J, Lundberg Jo, Holmgren A, et al
Proceedings of the National Academy of Sciences of the United States of America 2014;111(19):6964-9

APR-246/PRIMA-1MET inhibits thioredoxin reductase 1 and converts the enzyme to a dedicated NADPH oxidase
Peng X, Zhang Mq, Conserva F, Hosny G, Selivanova G, Bykov Vj, et al
Cell death & disease 2013;4():e881-

A Txnrd1-dependent metabolic switch alters hepatic lipogenesis, glycogen storage, and detoxification
Iverson Sv, Eriksson S, Xu J, Prigge Jr, Talago Ea, Meade Ta, et al
Free radical biology & medicine 2013;63():369-80

BBA General Subjects - Changing of the Guard at the Publishing Palace
Arner Esj
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2013;1830(6):III-IV

Multilevel regulation of 2-Cys peroxiredoxin reaction cycle by S-nitrosylation
Engelman R, Weisman-shomer P, Ziv T, Xu J, Arnér Es, Benhar M
The Journal of biological chemistry 2013;288(16):11312-24

Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping
Myers Jm, Cheng Q, Antholine We, Kalyanaraman B, Filipovska A, Arner Esj, et al
FREE RADICAL BIOLOGY AND MEDICINE 2013;:183-94

Selective activation of oxidized PTP1B by the thioredoxin system modulates PDGF-β receptor tyrosine kinase signaling
Dagnell M, Frijhoff J, Pader I, Augsten M, Boivin B, Xu J, et al
Proceedings of the National Academy of Sciences of the United States of America 2013;110(33):13398-403

Simvastatin inhibits the core promoter of the TXNRD1 gene and lowers cellular TrxR activity in HepG2 cells
Ekström L, Johansson M, Monostory K, Rundlöf Ak, Arnér Es, Björkhem-bergman L
Biochemical and biophysical research communications 2013;430(1):90-4

Site-specifically C-11-labeled Sel-tagged annexin A5 and a size-matched control for dynamic in vivo PET imaging of protein distribution in tissues prior to and after induced cell death
Cheng Q, Lu L, Grafstrom J, Olofsson Mh, Thorell Jo, Samen E, et al
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2013;1830(3):2562-73

The rare TXNRD1_v3 ("v3") splice variant of human thioredoxin reductase 1 protein is targeted to membrane rafts by N-acylation and induces filopodia independently of its redox active site integrity
Cebula M, Moolla N, Capovilla A, Arnér Es
The Journal of biological chemistry 2013;288(14):10002-11

Wobble decoding by the Escherichia coli selenocysteine insertion machinery
Xu J, Croitoru V, Rutishauser D, Cheng Q, Arnér Es
Nucleic acids research 2013;41(21):9800-11

Biochemical Discrimination between Selenium and Sulfur 1: A Single Residue Provides Selenium Specificity to Human Selenocysteine Lyase
Collins R, Johansson Al, Karlberg T, Markova N, Van Den Berg S, Olesen K, et al
PLOS ONE 2012;7(1):e30581-

Biochemical Discrimination between Selenium and Sulfur 2: Mechanistic Investigation of the Selenium Specificity of Human Selenocysteine Lyase
Johansson Al, Collins R, Arner Esj, Brzezinski P, Hogbom M
PLOS ONE 2012;7(1):e30528-

Combining [C-11]-AnxA5 PET Imaging with Serum Biomarkers for Improved Detection in Live Mice of Modest Cell Death in Human Solid Tumor Xenografts
Sel-tag Imaging Project, Cheng Q, Lu L, Grafstrom J, Olofsson Mh, Thorell Jo, et al
PLOS ONE 2012;7(8):e42151-

Hepatocyte DNA replication in growing liver requires either glutathione or a single allele of txnrd1
Prigge Jr, Eriksson S, Iverson Sv, Meade Ta, Capecchi Mr, Arner Esj, et al
FREE RADICAL BIOLOGY AND MEDICINE 2012;52(4):803-10

HER2-Positive Tumors Imaged Within 1 Hour Using a Site-Specifically C-11-Labeled Sel-Tagged Affibody Molecule
Wallberg H, Grafstrom J, Cheng Q, Lu L, Ahlzen Hsm, Samen E, et al
JOURNAL OF NUCLEAR MEDICINE 2012;53(9):1446-53

Pyrroloquinoline quinone modulates the kinetic parameters of the mammalian selenoprotein thioredoxin reductase 1 and is an inhibitor of glutathione reductase
Xu Jq, Arner Esj
BIOCHEMICAL PHARMACOLOGY 2012;83(6):815-20

Thiophosphate and selenite conversely modulate cell death induced by glutathione depletion or cisplatin: effects related to activity and Sec contents of thioredoxin reductase
Peng Xx, Xu Jq, Arner Esj
BIOCHEMICAL JOURNAL 2012;:167-74

Thioredoxin Reductase Inhibition Elicits Nrf2-Mediated Responses in Clara Cells: Implications for Oxidant-Induced Lung Injury
Locy Ml, Rogers Lk, Prigge Jr, Schmidt Ee, Arner Esj, Tipple Te
ANTIOXIDANTS & REDOX SIGNALING 2012;17(10):1407-16

Effects of selenite and chelating agents on mammalian thioredoxin reductase inhibited by mercury: implications for treatment of mercury poisoning
Carvalho Cml, Lu J, Zhang X, Arner Esj, Holmgren A
FASEB JOURNAL 2011;25(1):370-81

Human Protein Atlas of redox systems - What can be learnt?
Dammeyer P, Arner Esj
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2011;1810(1):111-38

Inhibition of thioredoxin reductase 1 by porphyrins and other small molecules identified by a high-throughput screening assay
Prast-nielsen S, Dexheimer Ts, Schultz L, Stafford Wc, Cheng Q, Xu Jq, et al
FREE RADICAL BIOLOGY AND MEDICINE 2011;50(9):1114-23

Redox Pioneer: Professor Arne Holmgren
Arner Esj
ANTIOXIDANTS & REDOX SIGNALING 2011;15(3):845-51

Selenoprotein TRXR-1 and GSR-1 are essential for removal of old cuticle during molting in Caenorhabditis elegans
Stenvall J, Fierro-gonzalez Jc, Swoboda P, Saamarthy K, Cheng Q, Cacho-valadez B, et al
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2011;108(3):1064-9

Substrate and inhibitor specificities differ between human cytosolic and mitochondrial thioredoxin reductases: Implications for development of specific inhibitors
Rackham O, Shearwood Amj, Thyer R, Mcnamara E, Davies Smk, Callus Ba, et al
FREE RADICAL BIOLOGY AND MEDICINE 2011;50(6):689-99

Noble metal targeting of thioredoxin reductase - covalent complexes with thioredoxin and thioredoxin-related protein of 14 kDa triggered by cisplatin
Prast-nielsen S, Cebula M, Pader I, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2010;49(11):1765-78

Selenoproteins-What unique properties can arise with selenocysteine in place of cysteine?
Arner Esj
EXPERIMENTAL CELL RESEARCH 2010;316(8):1296-303

The Interactions of Thioredoxin Reductase with Quinones and Acrolein: Covalent Adducts and Stimulation of NADPH Oxidase Activity
Myers Cr, Cheng Q, Myers Jm, Antholine We, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2010;:S98-S99

The Selenium-independent Inherent Pro-oxidant NADPH Oxidase Activity of Mammalian Thioredoxin Reductase and Its Selenium-dependent Direct Peroxidase Activities
Cheng Q, Antholine We, Myers Jm, Kalyanaraman B, Arner Esj, Myers Cr
JOURNAL OF BIOLOGICAL CHEMISTRY 2010;285(28):21708-23

Cisplatin and Oxaliplatin Toxicity: Importance of Cochlear Kinetics as a Determinant for Ototoxicity
Hellberg V, Wallin I, Eriksson S, Hernlund E, Jerremalm E, Berndtsson M, et al
JOURNAL OF THE NATIONAL CANCER INSTITUTE 2009;101(1):37-47

Crystal Structure and Catalysis of the Selenoprotein Thioredoxin Reductase 1
Cheng Q, Sandalova T, Lindqvist Y, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2009;284(6):3998-4008

Focus on mammalian thioredoxin reductases - Important selenoproteins with versatile functions
Arner Esj
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2009;1790(6):495-526

High levels of thioredoxin reductase 1 modulate drug-specific cytotoxic efficacy
Eriksson Se, Prast-nielsen S, Flaberg E, Szekely L, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2009;47(11):1661-71

Highly active dimeric and low-activity tetrameric forms of selenium-containing rat thioredoxin reductase 1
Rengby O, Cheng Q, Vahter M, Jornvall H, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2009;46(7):893-904

p53-dependent inhibition of TrxR1 contributes to the tumor-specific induction of apoptosis by RITA
Hedstrom E, Eriksson S, Zawacka-pankau J, Arner Esj, Selivanova G
CELL CYCLE 2009;8(21):3584-91

Prolonged antigen-exposure with carbohydrate particle based vaccination prevents allergic immune responses in sensitized mice
Thunberg S, Neimert-andersson T, Cheng Q, Wermeling F, Bergstrom U, Swedin L, et al
ALLERGY 2009;64(6):919-26

Red wine triggers cell death and thioredoxin reductase inhibition: Effects beyond resveratrol and SIRT1
Wallenborg K, Vlachos P, Eriksson S, Huijbregts L, Arner Esj, Joseph B, et al
EXPERIMENTAL CELL RESEARCH 2009;315(8):1360-71

Special issue on selenoprotein expression and function Preface
Lillig Ch, Arner E
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2009;1790(11):1387-8

Structure Mechanism Insights and the Role of Nitric Oxide Donation Guide the Development of Oxadiazole-2-Oxides as Therapeutic Agents against Schistosomiasis
Rai G, Sayed Aa, Lea Wa, Luecke Hf, Chakrapani H, Prast-nielsen S, et al
JOURNAL OF MEDICINAL CHEMISTRY 2009;52(20):6474-83

The human thioredoxin reductase-1 splice variant TXNRD1_v3 is an atypical inducer of cytoplasmic filaments and cell membrane filopodia
Damdimopoulou Pe, Miranda-vizuete A, Arner Esj, Gustafsson Ja, Damdimopoulos Ae
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 2009;1793(10):1588-96

Cell Death by SecTRAPs: Thioredoxin Reductase as a Prooxidant Killer of Cells
Anestal K, Prast-nielsen S, Cenas N, Arner Esj
PLOS ONE 2008;3(4):e1846-

Induction of cell membrane protrusions by the N-terminal glutaredoxin domain of a rare splice variant of human thioredoxin reductase 1
Dammeyer P, Damdimopoulos Ae, Nordman T, Jimenez A, Miranda-vizuete A, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2008;283(5):2814-21

Mitochondrial peroxiredoxin 3 is rapidly oxidized in cells treated
Brown Kk, Eriksson Se, Arner Esj, Hampton Mb
FREE RADICAL BIOLOGY AND MEDICINE 2008;45(4):494-502

The thioredoxin reductase inhibitor auranofin triggers apoptosis through a Bax/Bak-dependent process that involves peroxiredoxin 3 oxidation
Cox Ag, Brown Kk, Arner Esj, Hampton Mb
BIOCHEMICAL PHARMACOLOGY 2008;76(9):1097-109

Differential regulation of expression of cytosolic and mitochondrial thioredoxin reductase in rat liver and kidney
Crosley Lk, Meplan C, Nicol F, Rundlof Ak, Arner Esj, Hesketh Je, et al
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 2007;459(2):178-88

Thioredoxin glutathione reductase from schistosoma mansoni: An essential parasite enzyme and a key drug target
Kuntz An, Davioud-charvet E, Sayed Aa, Califf Ll, Dessolin J, Arner Esj, et al
PLOS MEDICINE 2007;4(6):1071-1086

Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug target
Kuntz An, Davioud-charvet E, Sayed Aa, Califf Ll, Dessolin J, Arnér Es, et al
PLoS medicine 2007;4(6):e206-

Titration and conditional knockdown of the prfB gene in Escherichia coli: Effects on growth and overproduction of the recombinant mammalian selenoprotein thioredoxin reductase
Rengby O, Arner Esj
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2007;73(2):432-41

Interactions of nitroaromatic compounds with the mammalian selenoprotein thioredoxin reductase and the relation to induction of apoptosis in human cancer cells
Cenas N, Prast S, Nivinskas H, Sarlauskas J, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2006;281(9):5593-603

Selenolthiol and dithiol C-terminal tetrapeptide motifs for one-step purification and labeling of recombinant proteins produced in E. coli
Cheng Q, Johansson L, Thorell Jo, Fredriksson A, Samen E, Stone-elander S, et al
CHEMBIOCHEM 2006;7(12):1976-81

Studies of an active site mutant of the selenoprotein thioredoxin reductase: The Ser-Cys-Cys-Ser motif of the insect orthologue is not sufficient to replace the Cys-Sec dyad in the mammalian enzyme
Johansson L, Arscott Ld, Ballou Dp, Charles Hw, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2006;41(4):649-56

Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with C-11
Cheng Q, Stone-elander S, Arner Esj
NATURE PROTOCOLS 2006;1(2):604-13

The thioredoxin system in cancer
Arner Esj, Holmgren A
SEMINARS IN CANCER BIOLOGY 2006;16(6):420-6

The thioredoxin system in cancer - iniroduction to a thematic volume of Seminars in Cancer Biology
Arner Esj, Holmgren A
SEMINARS IN CANCER BIOLOGY 2006;16(6):419-

A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation
Johansson L, Svensson L, Bergstrom U, Jacobsson-ekman G, Arner Esj, Van Hage M, et al
FEBS JOURNAL 2005;272(13):3449-60

EVALUATION OF NOVEL SELENOLTHIOL AND DITHIOL SEL-TAG VARIANTS FOR PURIFICATION AND C-11-LABELING OF RECOMBINANT PROTEINS PRODUCED IN E. COLI
Cheng Q, Johansson L, Fredriksson A, Thorell J, Stone-elander S, Arner Esj
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS 2005;:S38-S38

Inhibition of thioredoxin reductase but not of glutathione reductase by the major classes of alkylating and platinum-containing anticancer compounds
Witte Ab, Anestal K, Jerremalm E, Ehrsson H, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2005;39(5):696-703

Selenocysteine in proteins - properties and biotechnological use
Johansson L, Gafvelin G, Arner Esj
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 2005;1726(1):1-13

Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis
Rengby O, Johansson L, Carlson La, Serini E, Vlamis-gardikas A, Karsnas P, et al
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2004;70(9):5159-67

Evidence for intriguingly complex transcription of human thioredoxin reductase 1
Rundlof Ak, Janard M, Miranda-vizuete A, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2004;36(5):641-56

Exploiting the 21st amino acid - purifying and labeling proteins by selenolate targeting
Johansson L, Chen Cy, Thorell Jo, Fredriksson A, Stone-elander S, Gafvelin G, et al
NATURE METHODS 2004;1(1):61-6

Expression of selenocysteine-containing glutathione S-transferase in Escherichia coli
Jiang Zh, Arner Esj, Mu Y, Johansson L, Shi Jm, Zhao Sq, et al
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2004;321(1):94-101

Interactions of quinones with thioredoxin reductase - A challenge to the antioxidant role of the mammalian selenoprotein
Cenas N, Nivinskas H, Anusevicius Z, Sarlauskas J, Lederer F, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2004;279(4):2583-92

Overexpression of enzymatically active human cytosolic and mitochondrial thioredoxin reductase in HEK-293 cells - Effect on cell growth and differentiation
Nalvarte I, Damdimopoulos Ae, Nystom C, Nordman T, Miranda-vizuete A, Olsson Jm, et al
JOURNAL OF BIOLOGICAL CHEMISTRY 2004;279(52):54510-7

Regulation of the mammalian selenoprotein thioredoxin reductase 1 in relation to cellular phenotype, growth, and signaling events
Rundlof Ak, Arner Esj
ANTIOXIDANTS & REDOX SIGNALING 2004;6(1):41-52

Thioredoxin reductase 1 is upregulated in atherosclerotic plaques: Specific induction of the promoter in human macrophages by oxidized low-density lipoproteins
Furman C, Rundlof Ak, Larigauderie G, Jaye M, Bricca G, Copin C, et al
FREE RADICAL BIOLOGY AND MEDICINE 2004;37(1):71-85

Active sites of thioredoxin reductases: Why selenoproteins?
Gromer S, Johansson L, Bauer H, Arscott Ld, Rauch S, Ballou Dp, et al
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2003;100(22):12618-23

Rapid induction of cell death by selenium-compromised thioredoxin reductase 1 but not by the fully active enzyme containing selenocysteine
Anestal K, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2003;278(18):15966-72

Regeneration of the antioxidant ubiquinol by lipoamide dehydrogenase, thioredoxin reductase and glutathione reductase
Nordman T, Xia L, Bjorkhem-bergman L, Damdimopoulos A, Nalvarte I, Arner Esj, et al
BIOFACTORS 2003;18(1-4):45-50

The mammalian cytosolic selenoenzyme thioredoxin reductase reduces ubiquinone - A novel mechanism for defense against oxidative stress
Xia L, Nordman T, Olsson Jm, Damdimopoulos A, Bjorkhem-bergman L, Nalvarte I, et al
JOURNAL OF BIOLOGICAL CHEMISTRY 2003;278(4):2141-6

Recombinant expression of mammalian selenocysteine-containing thioredoxin reductase and other selenoproteins in Escherichia coli
Arner Esj
PROTEIN SENSORS AND REACTIVE OXYGEN SPECIES, PT A, SELENOPROTEINS AND THIOREDOXIN 2002;:226-35

Analysis of the inhibition of mammalian thioredoxin, thioredoxin reductase, and glutaredoxin by cis-diamminedichloroplatinum (II) and its major metabolite, the glutathione-platinum complex
Arner Esj, Nakamura H, Sasada T, Yodoi J, Holmgren A, Spyrou G
FREE RADICAL BIOLOGY AND MEDICINE 2001;31(10):1170-8

Measurement of thioredoxin and thioredoxin reductase
Arnér Es, Holmgren A
Current protocols in toxicology 2001;Chapter 7():7.4.-

Reactive oxygen species, antioxidants, and the mammalian thioredoxin system
Nordberg J, Arner Esj
FREE RADICAL BIOLOGY AND MEDICINE 2001;31(11):1287-312

The core promoter of human thioredoxin reductase 1
Rundlof Ak, Carlsten M, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 2001;276(32):30542-51

Physiological functions of thioredoxin and thioredoxin reductase
Arner Esj, Holmgren A
EUROPEAN JOURNAL OF BIOCHEMISTRY 2000;267(20):6102-9

Prominent expression of the selenoprotein thioredoxin reductase in the medullary rays of the rat kidney and thioredoxin reductase mRNA variants differing at the 5 ' untranslated region
Rundlof Ak, Carlsten M, Giacobini Mmj, Arner Esj
BIOCHEMICAL JOURNAL 2000;:661-8

Structure and mechanism of mammalian thioredoxin reductase: The active site is a redox-active selenolthiol/selenenylsulfide formed from the conserved cysteine-selenocysteine sequence
Zhong Lw, Arner Esj, Holmgren A
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2000;97(11):5854-9

Truncated thioredoxin is a mitogenic cytokine for resting human peripheral blood mononuclear cells and is present in human plasma
Pekkari K, Gurunath R, Arner Esj, Holmgren A
JOURNAL OF BIOLOGICAL CHEMISTRY 2000;275(48):37474-80

High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes
Arner Esj, Sarioglu H, Lottspeich F, Holmgren A, Bock A
JOURNAL OF MOLECULAR BIOLOGY 1999;292(5):1003-16

Preparation and assay of mammalian thioredoxin and thioredoxin reductase
Arner Esj, Zhong Lw, Holmgren A
OXIDANTS AND ANTIOXIDANTS, PT B 1999;:226-39

Superoxide production by dinitrophenyl-derivatized thioredoxin reductase - a model for the mechanism and correlation to immunostimulation by dinitrohalobenzenes
Arner Esj
BIOFACTORS 1999;10(2-3):219-26

Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue
Nordberg J, Zhong L, Holmgren A, Arner Esj
JOURNAL OF BIOLOGICAL CHEMISTRY 1998;273(18):10835-42

Rat and calf thioredoxin reductase are homologous to glutathione reductase with a carboxyl-terminal elongation containing a conserved catalytically active penultimate selenocysteine residue
Zhon Lw, Arner Esj, Ljung J, Aslund F, Holmgren A
JOURNAL OF BIOLOGICAL CHEMISTRY 1998;273(15):8581-91

Efficient reduction of lipoamide and lipoic acid by mammalian thioredoxin reductase
Arner Esj, Nordberg J, Holmgren A
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1996;225(1):268-74

On the phosphorylation of 2-chlorodeoxyadenosine (CdA) and its correlation with clinical response in leukemia treatment
Arner Esj
LEUKEMIA & LYMPHOMA 1996;21(3-4):225-31

1-CHLORO-2,4-DINITROBENZENE IS AN IRREVERSIBLE INHIBITOR OF HUMAN THIOREDOXIN REDUCTASE - LOSS OF THIOREDOXIN DISULFIDE REDUCTASE-ACTIVITY IS ACCOMPANIED BY A LARGE INCREASE IN NADPH OXIDASE ACTIVITY
Arner Esj, Bjornstedt M, Holmgren A
JOURNAL OF BIOLOGICAL CHEMISTRY 1995;270(8):3479-82

EXPRESSION OF DEOXYCYTIDINE KINASE AND PHOSPHORYLATION OF 2-CHLORODEOXYADENOSINE IN HUMAN NORMAL AND TUMOR-CELLS AND TISSUES
Spasokoukotskaja T, Arner Esj, Brosjo O, Gunven P, Juliusson G, Liliemark J, et al
EUROPEAN JOURNAL OF CANCER 1995;31A(2):202-8

MAMMALIAN DEOXYRIBONUCLEOSIDE KINASES
Arner Esj, Eriksson S
PHARMACOLOGY & THERAPEUTICS 1995;67(2):155-86

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