Björn Önfelt

About me
Publications
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About me

Björn Önfelt is Visiting Professor of Molecular Immunology with focus on
Cellular Immunotherapy at the Department of Medicine, Huddinge, Karolinska
Institutet.

Articles

Article: BONE MARROW TRANSPLANTATION. 2019;54(10):1562-1574

T-cell frequencies of CD8+ γδ and CD27+ γδ cells in the stem cell graft predict the outcome after allogeneic hematopoietic cell transplantation.

Gaballa A; Stikvoort A; Önfelt B; Mattsson J; Sundin M; Watz E; Uhlin M
Article: FRONTIERS IN IMMUNOLOGY. 2019;10:189

Individualization of Hematopoietic Stem Cell Transplantation Using Alpha/Beta T-Cell Depletion.

Rådestad E; Sundin M; Törlén J; Thunberg S; Önfelt B; Ljungman P; Watz E; Mattsson J; Uhlin M
Article: JOURNAL OF IMMUNOLOGY. 2018;201(7):2028-2041

Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1.

Walwyn-Brown K; Guldevall K; Saeed M; Pende D; Önfelt B; MacDonald AS; Davis DM
Article: JOURNAL OF CELL BIOLOGY. 2018;217(9):3267-3283

Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells.

Srpan K; Ambrose A; Karampatzakis A; Saeed M; Cartwright ANR; Guldevall K; De Matos GDSC; Önfelt B; Davis DM
Article: LAB ON A CHIP. 2018;18(16):2466-2476

Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging.

Olofsson K; Carannante V; Ohlin M; Frisk T; Kushiro K; Takai M; Lundqvist A; Önfelt B; Wiklund M
Article: BLOOD ADVANCES. 2018;2(12):1459-1469

161533 TriKE stimulates NK-cell function to overcome myeloid-derived suppressor cells in MDS.

Sarhan D; Brandt L; Felices M; Guldevall K; Lenvik T; Hinderlie P; Curtsinger J; Warlick E; Spellman SR; Blazar BR; Weisdorf DJ; Cooley S; Vallera DA; Önfelt B; Miller JS
Article: CANCER IMMUNOLOGY RESEARCH. 2018;6(4):467-480

Intrinsic Functional Potential of NK-Cell Subsets Constrains Retargeting Driven by Chimeric Antigen Receptors.

Oei VYS; Siernicka M; Graczyk-Jarzynka A; Hoel HJ; Yang W; Palacios D; Almåsbak H; Bajor M; Clement D; Brandt L; Önfelt B; Goodridge J; Winiarska M; Zagozdzon R; Olweus J; Kyte J-A; Malmberg K-J
Article: TRANSPLANTATION AND CELLULAR THERAPY. 2018;24(3):467-477

Risk Factors for Severe Acute Graft-versus-Host Disease in Donor Graft Composition.

Stikvoort A; Gaballa A; Solders M; Nederlof I; Önfelt B; Sundberg B; Remberger M; Sundin M; Mattsson J; Uhlin M
Article: SCIENTIFIC REPORTS. 2017;7(1):13433

Laboratory cryo x-ray microscopy for 3D cell imaging.

Fogelqvist E; Kördel M; Carannante V; Önfelt B; Hertz HM
Article: JOURNAL OF CELL BIOLOGY. 2016;215(6):875-889

NK cells converge lytic granules to promote cytotoxicity and prevent bystander killing.

Hsu H-T; Mace EM; Carisey AF; Viswanath DI; Christakou AE; Wiklund M; Önfelt B; Orange JS
Article: METHODS IN MOLECULAR BIOLOGY. 2016;1441:87-106

Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic Response.

Vanherberghen B; Frisk T; Forslund E; Olofsson PE; Guldevall K; Önfelt B
Article: FRONTIERS IN IMMUNOLOGY. 2016;7:119

Microchip Screening Platform for Single Cell Assessment of NK Cell Cytotoxicity.

Guldevall K; Brandt L; Forslund E; Olofsson K; Frisk TW; Olofsson PE; Gustafsson K; Manneberg O; Vanherberghen B; Brismar H; Kärre K; Uhlin M; Önfelt B
Article: JOURNAL OF IMMUNOLOGY. 2015;195(7):3374-3381

Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells.

Forslund E; Sohlberg E; Enqvist M; Olofsson PE; Malmberg K-J; Önfelt B
Article: LAB ON A CHIP. 2015;15(15):3222-3231

Ultrasonic three-dimensional on-chip cell culture for dynamic studies of tumor immune surveillance by natural killer cells.

Christakou AE; Ohlin M; Önfelt B; Wiklund M
Article: JOURNAL OF IMMUNOLOGY. 2015;194(9):4518-4527

Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.

Enqvist M; Ask EH; Forslund E; Carlsten M; Abrahamsen G; Béziat V; Andersson S; Schaffer M; Spurkland A; Bryceson Y; Önfelt B; Malmberg K-J
Article: FRONTIERS IN IMMUNOLOGY. 2015;6:196

Single-Cell Characterization of in vitro Migration and Interaction Dynamics of T Cells Expanded with IL-2 and IL-7.

Tauriainen J; Gustafsson K; Göthlin M; Gertow J; Buggert M; Frisk TW; Karlsson AC; Uhlin M; Önfelt B
Article: FRONTIERS IN IMMUNOLOGY. 2014;5:80

Distinct Migration and Contact Dynamics of Resting and IL-2-Activated Human Natural Killer Cells.

Olofsson PE; Forslund E; Vanherberghen B; Chechet K; Mickelin O; Ahlin AR; Everhorn T; Onfelt B
Article: INTEGRATIVE BIOLOGY. 2013;5(4):712-719

Live cell imaging in a micro-array of acoustic traps facilitates quantification of natural killer cell heterogeneity.

Christakou AE; Ohlin M; Vanherberghen B; Khorshidi MA; Kadri N; Frisk T; Wiklund M; Önfelt B
Article: JOURNAL OF MICROMECHANICS AND MICROENGINEERING. 2013;23(3):035008

Influence of acoustic streaming on ultrasonic particle manipulation in a 100-well ring-transducer microplate

Ohlin M; Christakou AE; Frisk T; Onfelt B; Wiklund M
Article: BLOOD. 2013;121(8):1326-1334

Classification of human natural killer cells based on migration behavior and cytotoxic response.

Vanherberghen B; Olofsson PE; Forslund E; Sternberg-Simon M; Khorshidi MA; Pacouret S; Guldevall K; Enqvist M; Malmberg K-J; Mehr R; Önfelt B
Article: FRONTIERS IN IMMUNOLOGY. 2013;4:65

Natural killer cell inhibitory receptor expression in humans and mice: a closer look.

Sternberg-Simon M; Brodin P; Pickman Y; Onfelt B; Kärre K; Malmberg K-J; Höglund P; Mehr R
Article: NANOSCALE. 2012;4(23):7383-7393

Visualization of custom-tailored iron oxide nanoparticles chemistry, uptake, and toxicity.

Wilkinson K; Ekstrand-Hammarström B; Ahlinder L; Guldevall K; Pazik R; Kępiński L; Kvashnina KO; Butorin SM; Brismar H; Önfelt B; Österlund L; Seisenbaeva GA; Kessler VG
Article: KIDNEY INTERNATIONAL. 2012;82(10):1071-1083

Plekhh2, a novel podocyte protein downregulated in human focal segmental glomerulosclerosis, is involved in matrix adhesion and actin dynamics.

Perisic L; Lal M; Hulkko J; Hultenby K; Önfelt B; Sun Y; Dunér F; Patrakka J; Betsholtz C; Uhlen M; Brismar H; Tryggvason K; Wernerson A; Pikkarainen T
Article: METHODS IN MOLECULAR BIOLOGY. 2012;853:177-196

Ultrasonic manipulation of single cells.

Wiklund M; Onfelt B
Article: FRONTIERS IN IMMUNOLOGY. 2012;3:300

Novel Microchip-Based Tools Facilitating Live Cell Imaging and Assessment of Functional Heterogeneity within NK Cell Populations.

Forslund E; Guldevall K; Olofsson PE; Frisk T; Christakou AE; Wiklund M; Onfelt B
Article: CHEMICAL COMMUNICATIONS. 2011;47(39):11020-11022

Light-induced cytotoxicity of a photochromic spiropyran.

Nilsson JR; Li S; Önfelt B; Andréasson J
Article: BIOMEDICAL MICRODEVICES. 2011;13(4):683-693

A silicon-glass microwell platform for high-resolution imaging and high-content screening with single cell resolution.

Frisk TW; Khorshidi MA; Guldevall K; Vanherberghen B; Önfelt B
Article: INTEGRATIVE BIOLOGY. 2011;3(7):770-778

Analysis of transient migration behavior of natural killer cells imaged in situ and in vitro.

Khorshidi MA; Vanherberghen B; Kowalewski JM; Garrod KR; Lindström S; Andersson-Svahn H; Brismar H; Cahalan MD; Önfelt B
Article: PLOS ONE. 2010;5(11):e15453

Imaging immune surveillance of individual natural killer cells confined in microwell arrays.

Guldevall K; Vanherberghen B; Frisk T; Hurtig J; Christakou AE; Manneberg O; Lindström S; Andersson-Svahn H; Wiklund M; Önfelt B
Article: LAB ON A CHIP. 2010;10(20):2727-2732

Ultrasound-controlled cell aggregation in a multi-well chip.

Vanherberghen B; Manneberg O; Christakou A; Frisk T; Ohlin M; Hertz HM; Önfelt B; Wiklund M
Article: LAB ON A CHIP. 2009;9(6):833-837

Flow-free transport of cells in microchannels by frequency-modulated ultrasound.

Manneberg O; Vanherberghen B; Onfelt B; Wiklund M
Article: BIOPHYSICAL JOURNAL. 2009;96(2):L13-L15

Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses.

Benninger RKP; Vanherberghen B; Young S; Taner SB; Culley FJ; Schnyder T; Neil MAA; Wüstner D; French PMW; Davis DM; Onfelt B
Article: APPLIED PHYSICS LETTERS. 2008;93(6):063901

A three-dimensional ultrasonic cage for characterization of individual cells

Manneberg O; Vanherberghen B; Svennebring J; Hertz HM; Onfelt B; Wiklund M
Article: NATURE CELL BIOLOGY. 2008;10(2):211-219

Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission.

Sowinski S; Jolly C; Berninghausen O; Purbhoo MA; Chauveau A; Köhler K; Oddos S; Eissmann P; Brodsky FM; Hopkins C; Onfelt B; Sattentau Q; Davis DM
Article: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2007;46(13):2228-2231

Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems.

Benninger RKP; Hofmann O; Onfelt B; Munro I; Dunsby C; Davis DM; Neil MAA; French PMW; de Mello AJ
Article: JOURNAL OF IMMUNOLOGY. 2007;178(6):3418-3426

The activating NKG2D ligand MHC class I-related chain A transfers from target cells to NK cells in a manner that allows functional consequences.

McCann FE; Eissmann P; Onfelt B; Leung R; Davis DM
Article: JOURNAL OF IMMUNOLOGY. 2006;177(12):8476-8483

Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria.

Onfelt B; Nedvetzki S; Benninger RKP; Purbhoo MA; Sowinski S; Hume AN; Seabra MC; Neil MAA; French PMW; Davis DM
Article: BIOPHYSICAL JOURNAL. 2005;88(1):609-622

Fluorescence imaging of two-photon linear dichroism: cholesterol depletion disrupts molecular orientation in cell membranes.

Benninger RKP; Onfelt B; Neil MAA; Davis DM; French PMW
Article: JOURNAL OF PHYSICS D-APPLIED PHYSICS. 2004;37(23):3296-3303

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy

Dunsby C; Lanigan PMP; McGinty J; Elson DS; Requejo-Isidro J; Munro I; Galletly N; McCann F; Treanor B; Önfelt B; Davis DM; Neil MAA; French PMW
Article: JOURNAL OF PHYSICAL CHEMISTRY A. 2004;108(20):4391-4398

Three-state light switch of [Ru(phen)₂dppz]²⁺:: Distinct excited-state species with two, one, or no hydrogen bonds from solvent

Olofsson J; Önfelt B; Lincoln P
Article: JOURNAL OF EXPERIMENTAL MEDICINE. 2004;199(7):1005-1010

Cell surface organization of stress-inducible proteins ULBP and MICA that stimulate human NK cells and T cells via NKG2D.

Eleme K; Taner SB; Onfelt B; Collinson LM; McCann FE; Chalupny NJ; Cosman D; Hopkins C; Magee AI; Davis DM
Article: JOURNAL OF PHYSICAL CHEMISTRY A. 2003;107(7):1000-1009

Picosecond and steady-state emission of [Ru(phen)₂dppz]²⁺ in glycerol:: Anomalous temperature dependence

Önfelt B; Olofsson J; Lincoln P; Nordén B
Article: MUTAGENESIS. 2002;17(4):317-320

Cell studies of the DNA bis-intercalator Delta-Delta [mu-C4(cpdppz)(2)-(phen)(4)Ru(2)](4+): toxic effects and properties as a light emitting DNA probe in V79 Chinese hamster cells.

Onfelt B; Göstring L; Lincoln P; Nordén B; Onfelt A
Article: JOURNAL OF PHYSICAL CHEMISTRY B. 2001;105(50):12653-12664

Picosecond time-resolved resonance Raman probing of the light-switch states of [Ru(Phen)₂dppz]²⁺

Coates CG; Olofsson J; Coletti M; McGarvey JJ; Önfelt B; Lincoln P; Norden B; Tuite E; Matousek P; Parker AW
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 2001;123(16):3630-3637

Enantioselective DNA threading dynamics by phenazine-linked.

Onfelt B; Lincoln P; Nordén B
Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2000;97(11):5708-5713

Femtosecond linear dichroism of DNA-intercalating chromophores: solvation and charge separation dynamics of [Ru(phen)2dppz]2+ systems.

Onfelt B; Lincoln P; Nordén B; Baskin JS; Zewail AH
Article: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 1999;121(46):10846-10847

A molecular staple for DNA:: Threading bis-intercalating [Ru(phen)₂dppz]²⁺ dimer

Önfelt B; Lincoln P; Nordén B
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All other publications

Review: IMMUNOLOGICAL REVIEWS. 2015;267(1):197-213

Newtonian cell interactions shape natural killer cell education.

Goodridge JP; Önfelt B; Malmberg K-J
Review: MICROMACHINES. 2014;5(1):27-49

Ultrasound-Induced Cell-Cell Interaction Studies in a Multi-Well Microplate

Wiklund M; Christakou AE; Ohlin M; Iranmanesh I; Frisk T; Vanherberghen B; Onfelt B
Review: FEBS JOURNAL. 2013;280(12):2775-2784

Consequences of membrane topography.

Parmryd I; Onfelt B
Editorial comment: LAB ON A CHIP. 2012;12(18):3221-3234

Acoustofluidics 18: Microscopy for acoustofluidic micro-devices.

Wiklund M; Brismar H; Onfelt B
Review: WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY. 2010;2(3):260-276

Intercellular nanotubes: insights from imaging studies and beyond.

Hurtig J; Chiu DT; Onfelt B
Corrigendum: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2007;46(45):8536

Fluorescence-lifetime imaging of DNA-Dye interactions within continuous-flow microfluidic systems (vol 46, pg 8525, 2007)

Benninger RKP; Hofmann O; Onfelt B; Munro I; Dunsby C; Davis DM; Neil MAA; French PMW; de Mello AJ
Review: SCIENCES STKE [ELECTRONIC RESOURCE] : SIGNAL TRANSDUCTION KNOWLEDGE ENVIRONMENT. 2005;2005(313):pe55

Long-distance calls between cells connected by tunneling nanotubules.

Onfelt B; Purbhoo MA; Nedvetzki S; Sowinski S; Davis DM
Published conference paper: BIOCHEMICAL SOCIETY TRANSACTIONS. 2004;32(Pt 5):676-678

Can membrane nanotubes facilitate communication between immune cells?

Onfelt B; Davis DM
Review: TRAFFIC. 2004;5(9):651-661

Control of immune responses by trafficking cell surface proteins, vesicles and lipid rafts to and from the immunological synapse.

Taner SB; Onfelt B; Pirinen NJ; McCann FE; Magee AI; Davis DM
Published conference paper: JOURNAL OF IMMUNOLOGY. 2004;173(3):1511-1513

Cutting edge: Membrane nanotubes connect immune cells.

Onfelt B; Nedvetzki S; Yanagi K; Davis DM
Published conference paper: JOURNAL OF INORGANIC BIOCHEMISTRY. 2002;91(1):286-297

Picosecond Kerr-gated time-resolved resonance Raman spectroscopy of the [Ru(phen)(2)dppz](2+) interaction with DNA.

Olofsson J; Onfelt B; Lincoln P; Nordén B; Matousek P; Parker AW; Tuite E
Published conference paper: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. 2000;17 Suppl 1:277-283

Probing DNA conductivity with photoinduced electron transfer and scanning tunneling microscopy.

Tuite E; Lincoln P; Olofsson J; Becker HC; Onfelt B; Erts D; Nordén B

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