Real Time PCR

There are two Real-Time PCR systems at CCK. Both require training and booking. Please, see below for details.

Location: CCK, floor 03 room 057

Manufacturer: Applied Biosystems
Model: 7500 Real-Time PCR System

This Real-Time PCR system is used for analyses of gene expression, allele/mutation specific PCR or deletion analyses (LOH). The excitation is by a halogen lamp and the fluorescent signal is detected by a CCD camera.

Booking of the instrument - maximum 3 h for one run and analysis; the booked time may be lost if you miss the starting time point by more than 30 minutes.

All samples for Applied Biosystems 7500 Real-Time PCR system should be prepared on half-skirted 96-well plates with adhesive film (Applied Biosystems plate 10-pack part number N801-0560 and 25-pack MicroAmp Optical Adhesive Film 4360954 or Sarstedt order numbers plates 25/bag 100/case 72.1979.202 and Adhesive Sealing Tape 100/package order number 95.1994). The used plate is identical to that used in the ABI3130xl Genetic Analyzer (Sequencer).

The software is different from that of the Applied Biosystems 7900HT Fast Real-Time PCR system.

Location: CCK, floor 03 rum 057

Manufacturer: Applied Biosystems
Model: 7900HT Fast Real-Time PCR system

This Real-Time PCR system is used for analyses of gene expression and medium high-throughput genotyping. It differs from the 7500 machine by having a 384-well and fast block options (shorter total time for PCR) and by having excitation by laser/detection well-by-well through an optical cable. The software is also different compared to the 7500 machine.

Booking of the instrument - maximum 3 h for one run and analysis; booked time may be lost if you miss the starting time point by more than 30 minutes.

All samples for Applied Biosystems 7900HT Fast Real-Time PCR System should be prepared on half-skirted 96-well plates with adhesive film (Applied Biosystems 20 plates MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1mL part number 4346906 and 25-pack MicroAmp Optical Adhesive Film 4360954 or Optical 384-well reaction Plate part 4309849 (50 pieces).

Important message to all the users of the 7900HT Fast Real-Time PCR system!

The experiments on the 96-well FAST blocks can be performed during Monday-Wednesday (18.00). The 384-well plates should be run on Thursday-Friday (+ weekend if required). Please, contact Rainer Tuominen (073 7334 231) in case of emergency.

Booking of the PCR machine

(User group CCK qPCR (7900HT [96/384]))

Contact persons for changing the blocks

Carina Strell

Jason Serviss

Matheus Dyczynski

Sander Busker