Karolinska High Throughput Center

Karolinska High Throughput Center

Introduction to KHTC

KHTC offers open access to one of the most sophisticated and versatile analysis platforms in Europe, which includes integrated liquid and microplate handling, high-throughput screening (HTS) and Next Generation Sequencing (NGS) capabilities. We provide services, expertise and training in the fields of systems biology, functional genomics and drug-discovery to the Swedish and international scientific community. KHTC is part of the national SciLifeLab Functional Genomics platform.


  • Assay: miniaturization and optimization to microplate formats
  • Liquid handling: down to nl levels in 96-, 384- and 1536-microplate formats
  • HTS: screening of compound or small interfering RNAs (siRNA) libraries in biochemical and cellular assays including hit-validation or follow up of knock-down efficiency
  • NGS: assistance and access to sequencing capabilities with rapid turnaround times
  • Training: experimental design and operation of the high-throughput laboratory platforms

Equipment and tools

Technological platforms:

  • The BIG robot - flexible assay development and HTS platform containing 28 integrated instruments
  • High-throughput flow cytometry platform with integrated auto-sampler system
  • High-capacity platform for analysis of biomolecular interactions
  • Pre-PCR DNA barcoding platform
  • Microbial colony picking and arraying systems
  • Next Generation Sequencing platform

Detection instruments:

  • Automated microscope: Pathway 855 from BD Biosciences
  • High-content imager: Acumen eX3 from TTP Labtech
  • Flow cytometer; CyAn ADP from Beckman
  • Multilabel plate reader; EnVision from PerkinElmer
  • Real-time PCR device; LightCycler 480 from Roche
  • NGS sequencers; HiSeq2000 from Illumina


  • Compound collection
  • siRNA libraries
  • ORFeome clones

Facility updates

  • Altogether 26 projects were completed in 2014
  • We have assisted scientists from four Swedish universities, several international universities and also from small biotech companies
  • Results generated within the facility during 2010-2014 have been incorporated into19 publications, five of these articles have been published in journals with impact factor >25
  • During last year we received funding from Vinnova for collaboration with a Swedish biotech company, which allowed screening of the entire compound collection
  • We have recently expanded our services within small molecule screening to offer better analysis services using the tools from Collaborative Drug Discovery


Platform Director:
Jussi Taipale

Platform Manager:
Anders Eriksson

Laboratory Manager:
Jianping Liu

Research Engineer:
Natalia Nekhotiaeva

Senior Research Scientist:
Minna Taipale


Laboratory manager

Anders B Eriksson

Organizational unit: Department of Medical Biochemistry and Biophysics (MBB), C2
E-mail: anders.eriksson@ki.se



Systems Biology Group - Taipale lab

Core facilityDrugGene regulationGeneticsGenomicsImagingPharmacologySystems Biology