Phenotyping of mouse models at KI Mouse Clinic
Karolinska Institutet Mouse Clinic (KIMC) at Comparative Medicine offers the possibility to assist with phenotype analysis of genetically modified mice.
Phenotyping from Greek phainen (to show) and tupos (type) means in this context the detection of changes of observable features (like for example behavior, physiology) of genetically modified animals, based on the interaction of the genes of the animal. The changed phenotype reflects the influence of the gene on the organismal (animal) level.
Submit an enquiry online
Clients from Karolinska Institutet currently have the possibility to submit enquiries online for use of our services. Log on to the form using your KI ID. More information is available once you have logged in.
Clients who are affiliated with institutions outside Karolinska Institutet are welcome to contact us via email. For questions regarding the online enquiry service or for general queries, please find our contact details below.
Phenotyping at KIMC
The Mouse Clinic offers researchers the possibility to document the health of their genetically modified mouse strains according to international standards. This is preferably done through established phenotyping pipelines, the International Mouse Phenotyping Consortium (IMPC) and the German Mouse Clinic (GMC). You may also choose select phenotyping analyses. In order to submit an enquiry, please use the online enquiry links above.
Protocols are in accordance with the International Mouse Phenotyping Resource of Standardised Screens (IMPReSS) but may differ in some aspects.
Select phenotyping analyses
A range of select phenotyping analyses such as hematological-, physiological- and pathological and/or behavioural analyses are provided by the Mouse Clinic. Please read more about each service in the links below.
- Behavioural tests
- Physiological tests
- Imaging analyses
- Morphological analysis - pathology
- Hematology, urine chemistry and clinical blood chemistry
Performed in collaboration with either two partners:
1. Swedish Toxicology Sciences Research Center (SweTox) in Gärtuna,
Plasma and serum analyses are conducted with three fixed kits;
1. Comprehensive diagnostics (Alb, Alt, Alp, Amy, Tot Bil, BUN, Ca, Crea, Glob, Glu, Phos, K, Na, Tot Prot)
2. Kidney profile (Alb, Cl, Crea, Glu, Phos, K, Na, Tot CO2, BUN)
3. Mammalian liver profile (Alb, Alt, Alp, Bile Acids, Tot Bil, Chol, GGT, BUN).
This means that depending on your requirements you may need to combine two or three kits.
2. Clinical Chemistry Laboratory, University Animal Hospital in Uppsala
For more information on available parameters, please use a Clinical Chemistry Laboratory referral.
- Eye morphology
Eye morphology is performed in collaboration with Sankt Erik Eye Hospital in Stockholm. Examination is performed in line with the IMPRESS protocols, except for the following exceptions: Examination using Optical Coherence Tomography (OCT), Pentacam or Scheimpflug images cannot be performed.
Performed in collaboraton with Swedish Toxicology Sciences Research Center in Gärtuna
The flow cytometry facility at Swetox, Södertälje, offers a broad range of immunophenotypic analyses. A standard (IMPReSS) panel is listed below but custom-made analyses can be applied and is only limited by access to relevant tracer antibody. For cell-assays, spleen and/or peripheral blood can be used as sources. For cytokine profiling, serum/cell-supernatants can be analysed. SweTox also offers expert advice.
Price list (pdf)
|Address:||KIMM, Comparative Medicine, Karolinska Institutet, SE-171 77 Stockholm, Sweden|