Karolinska Institutet Zebrafish Core Facility
The Karolinska Institutet Zebrafish Core Facility is open to all researchers wishing to use the zebrafish as an experimental model.
The zebrafish (Danio rerio) is a popular laboratory animal for a number of reasons. Zebrafish are vertebrates and they are easy to keep. The females produce large clutches of eggs and the embryos develop very quickly, with major organs in place by six days post-fertilisation. Since the embryos develop outside the mother and are transparent, they offer an opportunity to follow the formation of tissues and organs from a very early stage onwards.
There are already zebrafish models for a number of human diseases, for example cardiomyopathy, diabetes, muscular dystrophy and Parkinson's disease.
Activities and services
The state-of-the-art facility at the Karolinska Institutet Zebrafish Core Facility - the largest facility in Sweden for zebrafish - houses fish in a continuously monitored environment and all staff are qualified animal technicians. We can provide researchers with fish from wildtype lines, as well as offer the possibility of bringing in mutant/transgenic fish lines of interest from other laboratories.
The facility has, in collaboration with researchers from Karolinska Institutet, developed protocols for a wide range of techniques, including morpholino injections, whole mount in situ hybridisation, electron microscopy and immunofluorescence assays.
Users of the facility have the following equipment at their disposal:
- Eppendorf FemtoJet Microinjectors
- Leica MZ16F Fluorescence Stereomicroscope
- Leica DFC320 Digital FireWire Color Camera System
- Leica M165FC Fluorescence Stereomicroscope
- Leica DFC450C Digital FireWire Color Camera System
- Nikon SMZ645 and SMZ800 Stereoscopic Zoom Microscopes
Contact and organisation
The zebrafish core facility is part of Comparative Medicine at Karolinska Institutet.
Monika Andersson Lendahl
Phone: +46 8-524 872 18