Biophysics of stem cell and tissue growth
Research in our group aims for a deeper understanding of the factors that control self-renewal in stem cells.
Self-renewal is the capacity of a stem cell to maintain a stem cell identity while realizing its potential to differentiate. Self-renewal necessarily also entails maintained proliferative capacity. Understanding the factors that control proliferation is essential when developing regenerative therapies but also therapies against self-renewing tumor initiating cells (cancer stem cells) that underlie tumor relapse and metastasis.
We aim to identify factors that:
- induce the transition from quiescence to active proliferation in stem cells to allow tissue repair/engineering
- factors that protect stem cells during radio-/chemotherapy and
- factors that target the proliferative and metastatic capacity in cancer stem cells.
To this end we explore how biophysical mechanisms, involving membrane potential and electrochemical signaling, affect stem cell behavior and morphology associated with growth, proliferation and migration. We furthermore explore how photobiological and biophysical tools (e.g. optogenetics, ion channels) may integrate with these functions and aim to identify potential downstream effects on the cell intrinsic molecular clock and on metabolism.
We work with an experimental systems biology approach reaching from pharmacology, small molecule screens and genetic validations in stem cells, cancer stem cells and mouse and zebrafish models, extending to genomics (RNAseq) and a range of biophysical methods.
|Michael Andäng|| |
Research group leader
|Julianna Kele Olovsson||Associated|
Past group members:
Mia Niklasson, post-doc, present position senior researcher at Uppsala University.
Helena Johard, post-doc, co-supervisor of doctoral student Anna Omelyanenko.
1. Membrane potential and ion fluxes in control of:
- 1.1 the cell cycle in embryonic stem cells (Shaimaa Abdelhady).
- 1.2 the cell cycle in neural stem cells (Anna Omelyanenko, Fei Gao).
- 1.3 cell proliferation in glioma tumors and glioma cancer stem cells (Shermaine Wee).
- 1.4 embryo development (Petra Sekyrova).
- 1.5 molecular clock oscillations (Anna Omelyanenko).
2 Cell cycle dependent gene expression - from transcription factors to ion channels - in:
- 2.1 embryonic stem cells, induced pluripotent stem cells and cancer cells (Petra Sekyrova).
- 2.2 neural stem cells.
- 2.3 cancer stem cells (Shermaine Wee).
- 2.4 interactions with Epithelial to Mesenchymal Transition in G2 cell cycle phase (Petra Sekyrova).
3. Light and magnetic field controlled gene expression and function:
- 3.1 in control of basic biological mechanisms such as cell cycle and molecular clock oscillations (Petra Sekyrova, Anna Omelyanenko).
- 3.2 reengineered to experimentally control gene expression (all members of the group).
- Swedish Research Council
- Swedish Cancer Society
- Swedish Childhood Cancer Foundation
- Åke Wiberg Foundation (page in Swedish)
- Linnaeus Center in Developmental Biology for Regenerative Medicine
- Karolinska Institutet
Blebbing as a physical force in cancer EMT - parallels with mitosis.
Semin. Cancer Biol. 2012 Oct;22(5-6):369-73
Quiescence and γH2AX in neuroblastoma are regulated by ouabain/Na,K-ATPase.
Br. J. Cancer 2012 May;106(11):1807-15
Cell cycle restriction by histone H2AX limits proliferation of adult neural stem cells.
Proc. Natl. Acad. Sci. U.S.A. 2011 Apr;108(14):5837-42
Mouse embryonic stem cell-derived spheres with distinct neurogenic potentials.
Stem Cells Dev. 2008 Apr;17(2):233-43
Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation.
Nature 2008 Jan;451(7177):460-4
A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells.
Hum. Reprod. 2003 Jul;18(7):1404-9