Welcome to the CMB Core Infrastructures
The Core Infrastructures at CMB (KI) are an umbrella definition for service laboratories, resources and competence centers whose activities are coordinated, supervised and partly financed by the Board of Research (FoS) in collaboration with the local committees for Research and Education (FoUU) of the associated hospitals.
The aim of the Core Infrastructures efforts are to allow wide access to state of the art technologies, sample and data collections, informatics tools and scientific consultation in order to facilitate the interaction between researchers and enhance scientific productivity.
Access to the Core Infrastructures are open to all researchers at KI and other academic institutions and, to a limited extent, also to commercial enterprises. Service Laboratories and Resources operate on a fee per service basis while the Competence Centers aim to disseminate specific technologies and know how on a collaborative basis.
This page contains links to the Service Laboratories, Resources, and Competence Centers offered at CMB and that are available to members of the Karolinska Institutet research community at KI campus and associated Hospitals.
VirusTech Core Facility
Viruses have developed as useful tools for the efficient delivery of genes to mammalian cells. Thus it is no wonder that viruses have led the field in gene therapy, as they have evolved to deliver their genes efficiently, in the form of either RNA or DNA into mammalian cells. In research, delivery of genetic information to cellular systems is the backbone of many experiments. This becomes specially challenging in neuroscience where non-viral methods to incorporate RNA or DNA into the target cells (specially Neurons) are limited, and this turns out to be more difficult when applied to developmental biology. Thus, the ability of gene transfer into host cells, both in culture cells and in vivo organisms in different developmental stages, becomes crucial to understand the function of genes in the development and cellular processes such as stem cell maintenance, cell differentiation and maturation. Therefore, the VirusTech Core Facility has as its primary goal to help fellow researchers, to overcome such challenges in gene transfer by providing our services and knowledge in virus production and usage.
Albert Blanchart Aguado
Electron Tomography Facility
To understand the life processes it is necessary to know where the specific macromolecules are located within a cell and tissue and how the macromolecules interact with each other. Electron tomography permits subcellular and macromolecular assemblies to be studied in three dimensions; the molecular objects are reconstructed in three dimensions from a series of electron micrographs representing different projections of the objects.
The electron tomography facility at CMB is supported by central KI funds and the Stockholm County Council through the Joint Core-Facility Committee, and can be engaged by researchers at all departments as well as other universities and industry.
The CMB FACS (Fluorescence Activated Cell Sorter) facility is primarily intended for CMB researchers. External users are welcome when the facility is not utilized by CMB users.
The goal for the facility is to offer high speed/purity cell sorting and advanced flow cytometry analysis.
The facility is equipped with instruments for cell sorting and multicolor analysis.
For Cell Sorting
- BD FACSAria III Cell Sorter system with a four-laser configuration (violet 405nm, blue 488nm, yellow-green 561nm and red 633nm) and 11 fluorescence detectors, which allow multicolor detection and sorting.
For multicolor analysis
- FACSCanto II with a three laser configuration (405nm, 488nm and 633nm) and 8 fluorescence detectors.
- FACSCalibur with two laser configuration (488nm and 635nm) and 4 fluorescence detectors.
- Users require “certified driving license” for running samples.
|CMB Group |
|External Group, KI |
The CMB Microscope Facility offers the opportunity for internal and external users to perform fluorescence microscopy on various advanced systems dedicated for imaging of fixed tissues, real-time live cell imaging, and high content imaging experiments. The users can electronically book and use a microscope platform after an introduction and training session. The CMB Microscope Facility provides also assistance with experimental designs and image analyses, organizes annual live cell imaging PhD courses, and participates in other microscopy-oriented courses.
Microscope platforms available at the facility:
- Zeiss Confocal Microscope LSM880: scanning laser confocal microscope for imaging of fixed samples.
- Zeiss Confocal Microscope LSM510 META: scanning laser confocal microscope for imaging of fixed samples and living cells as well as bleaching- and photoactivation techniques and spectral analysis.
- DeltaVision Spectris Deconvolution Microscope: widefield deconvolution fluorescence microscope for imaging of fixed samples and living cells.
- Angstrom Grid Confocal Microscope: multifunctional widefield fluorescence microscope with a high-resolution confocal configuration based on Structural Illumination Microscopy (SIM). This system is also suited for time-lapse imaging and deconvolution microscopy and equipped with a motorized pulsed laser for micromanipulation.
- Leica DMI6000: automated live cell imaging system with minimal phototoxicity for long- time lapse experiments.
- ImageXpress Micro: automated imaging and analysis system for high throughput screening of fixed samples or living cells.
- Volocity Image Analysis: workstation for visualisation, quantification, and image restoration.