Equipment and Access

Access and Booking

Fees

Fees at the LCI Unit

The Live Cell Imaging facility is open to all researchers. Prices are listed below.

Users pay an entry fee once. This includes training as well as help with sample preparation, experimental design and image analysis. Additionally, users pay the usage fee, every year, 3 or 6 months depending on the length of their project. There is no additional fee per hour. The usage fee is the same regardless of the number of microscopes you use (after training).

The entry fee for the Nikon confocals gives access to 4 confocals (Tweety, Pegasus, Dragon and Orion).
The entry fee for the Nikon widefield systems gives access to 3 widefield systems (Orion, Victor and Tweety), 2 TIRF systems (Orion and Victor), STORM (Orion) and 1 spinning disk (Victor).

The entry fee for the Zeiss confocal gives access to 1 confocal with a multiphoton option (Zeus).

If one user has paid for one of the entry fees then needs to start using a different system, the new entry fee is cheaper (see table below).

Users of the microscopes have access to the 3D analysis software without any additional entry fee (Imaris and NIS).

1. Entry fee per user and per type of microscope or software (includes advanced training and expert assistance)

One-time entry fee, 1st type of microscope 12.000 SEK
One-time entry fee, 2nd type of microscope 9.000 SEK
One-time entry fee for Imaris and/or offlines only (non-microscope users) 5000 SEK

2. Usage fee

  12 months 6 months 3 months
Usage fee for all microscopes and offlines 16.500 SEK 10.000 SEK 6.500 SEK
Usage fee for Imaris and/or offlines only 5.000 SEK 3.500 SEK 2.000 SEK

For non academic users the yearly fee is double.

 

Registration and booking

Booking at LCI Unit

Fee form

Booking (log-in required)

 

Laser safety

Laser safety at the LCI unit

Lasers at the LCI facility microscopes fire visible or invisible light.  Invisible lasers are the 405 nm laser and the multiphoton laser.
Never look down the eyepiece when the system is scanning, even if you do not see any light!
Laser safety at the LCI facility

 

Guidlines

Guidelines LCI Unit

The LCI facility guidelines must be followed by all users.

 

Nikon Confocal and widefield (Tweety)

 

 

Specifications:

- Ti-E inverted point scanning confocal microscope A1+
- Galvanometric scanner
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um
- Hamamatsu sCMOS Orcha-Flash 4.0. 2048x2048 pixels. Pixel size 6.45 um. QE 82%

Wide Field          
Cube name     Excitation Filters Dichroic Mirrors Emission Filters
Blue
Green
Red
    387/11
472/30
562/40
409LP
513LP
601LP
447/60
536/40
624/40
Confocal    
Lasers Dichroic Mirrors Emission Filters

405 diode
488 diode
561 diode
640 diode

405/488
405/488/561
405/488/561/640
BS 20/80

450/50
525/50
595/50
700/75

Nikon Confocal with resonant scanner (Pegasus)

 

Specifications:

- Ti-E inverted point scanning confocal microscope A1R Si
- Galvanometric and resonant scanner
- 2x GaAsP Detectors (green and red),
- 1x32 channel Spectral Detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um

Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon Confocal with resonant scanner, spectral detector and FLIM (Dragon)

 

  

Specifications:

- Ti-E inverted point scanning confocal microscope A1R Si
- Galvanometric and resonant scanner
-  2x GaAsP detectors (green and red),
- 1x32 channel Spectral Detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z
- La Vision BioTech FLIM (Fluorescence Live time Microscopy)

Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon Confocal with spectral detector and TIRF, STORM and widefield (Orion)

 

Specifications:

- Ti-E inverted point scanning confocal microscope A1 Si
- Galvano scanner
- 1x32 channel Spectral detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um
- TIRF (Total Internal Reflection Fluorescence microscopy)
- STORM (Stochastic Optical Reconstruction microscopy)
- Andor EM-CCD camera, 512x512, pixel size 16um.

Wide Field        
Cube Excitation Filters   Dichroic Filters Emission Filters

Blue
Green
Red
Far Red

387/11
472/30
562/40
620/60
  409LP
495LP
593LP
660LP
447/60
520/35
624/40
700/75
         

STORM/TIRF

Cube Excitation Filters Dichroic Filters Emission Filters
Dual TIRF 480-495
555-565
495-545
565LP
500-545
570LP
Quad TIRF 385-418
485-495
555-570
635-660
420-480
495-555
575-630
670LP
422-480
500-550
580-625
675LP
STORM   405/5
488/5
561/5
647/5
670-750
Confocal    
Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode (300 mWatt)

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon spinning disk confocal, TIRF and widefield (Victor)

 

Specifications:

Nikon Ti-E spinning disk confocal or widefield microscope
Motorized XY stage
Motorized Z piezo stage and Z piezo 250 um
Full size incubator for life cell imaging

Special features: 2 possible configurations:

Spinning disk confocal

- Andor EM-CCD camera. 512x512 pixels. Pixel size 16 um. QE 95%
- Spinning disk Yokagawa X1, 10,000 rpm

Lasers for spinning disk imaging Quad Dichroic Mirror Emission Filters

405 nm diode
488 nm diode
561 nm diode
640 nm diode

425-480
503-555
580-628
675LP

447/60
512/30 ; 630/100
525/30
525 600 690
607/36
685/40

Widefield microscope

- Andor sCMOS Zyla 4.2+. 2048x2048 pixels. Pixel size 6.45 um. QE 82%
-SpectraX light source (six independently controllable light sources that span the visible spectrum from 380-680nm

LEDs from SpectraX for wide field imaging Dichroic Mirrors Emission Filters
395/25 nm
440/20 nm
475/28 nm
510/25 nm
550/15 nm (or 575/25)
631/28 nm
425-480
503-555
580-628
675LP
447/60
483/32
500LP
525/30
542/27
607/36
630/92
685/40

Zeiss single and multi-photon confocal (Zeuss)

 

Specifications:

- Zeiss point scanning confocal LSM710-NLO
- Upright microscope
- Motorized XY stage and Z
- Full size incubator for live cell imaging
- Spectral detector (descanned)
- Single and Two-photon laser
- Two Non-descanned detectors (Epi and Trans)
- GaAsP ultra sensitive detector

Lasers:
- 405nm diode
- 457nm/488nm/514nm Argon Ion
- 561nm diode
- 633nm diode

Objectives

Permanently available on each of our NIKON microscopes:

Magnification Type n NA WD mm FOV um
10x Dry 1 0.45 4 1257
20x Dry 1 0.75 1 629
60x Oil 1.55 1.40 0.13 314

Additional objectives rotating between our NIKON microscopes:

Magnification Type n NA WD mm FOV um
40x Dry 1 0.95 0.14-0.25 (ring) 314
40x Oil 1.52 1.30 0.22 (ring) 314
60x (TIRF) Oil 1.52 1.49 0.12 (ring) 210
60x Water 1.33 1.20 0.28-0.31(ring) 210
100x (TIRF) Oil 1.52 1.49 0.12 (ring) 126

Objectives available on Zeiss LSM710

Magnification Type n NA WD mm FOV um
5x Dry 1 0.16 18.5 1700
20x Dry 1 0.6 1.7 425
20x Dipping 1.33 1.00 1.8 425
40x Water 1,33 1.10 0.62 213
63x Water 1.33 1.20 0.28 135

Software

We have 4 offline stations at the LCI Facility equipped with the following softwares:

- NIS Element: offline software for the Nikon systems
- Zeiss Zen: offline software for Zeus
- Imspector Pro: offline software for FLIM
- Imaris: powerful 5D rendering and statistical analysis software
- Cell Profiler: 4D (no z) rendering and statistical analysis freeware
- ImageJ/Fiji: image processing and visualizing freeware
- XUV Tools: 3D image alignment freeware
- MatLab: custom-made analysis

Microscopy