Equipment and Access

Access and Booking

Fees

Fees at the LCI Unit

The Live Cell Imaging facility is open to all researchers. Prices are listed below.

Users pay an entry fee once. This includes training as well as help with sample preparation, experimental design and image analysis. Additionally, users pay the usage fee, every year, 3 or 6 months depending on the length of their project.

The entry fee for the Nikon confocals gives access to 4 confocals (Tweety, Pegasus, Dragon and Orion). The entry fee for the Nikon widefield systems gives access to TIRF on Orion and TIRF, widefield or spinning disk on Victor. Finally there is an entry fee for the Zeiss confocal (Zeus) which gives access to one confocal with a multiphoton option. If one user has paid for one of the entry fees then needs to start using a different system, the new entry fee is cheaper (see table below).

1. Entry fee per user and per microscope or software (includes advanced training and expert assistance)

Entry fee for first training 12.000 SEK
Entry fee for subsequent training 9.000 SEK
Entry fee for Imaris or NIS (offline PC only) 5.000 SEK

2. Usage fee
There is no additional fee per hour. The usage fee is the same regardless on the number of microscopes you use (after training).

  12 months 6 months 3 months
Offline software only 4.000 SEK 2.500 SEK 1.500 SEK
Microscopes and offlines 15.000 SEK 9.000 SEK 6.000 SEK

For non academic users the yearly fee is 30,000 SEK.

 

Registration and booking

Booking at LCI Unit

Fee form

Booking (log-in required)

 

Laser safety

Laser safety at the LCI unit

Lasers at the LCI facility microscopes fire visible or invisible light.  Invisible lasers are the 405 nm laser and the multiphoton laser.
Never look down the eyepiece when the system is scanning, even if you do not see any light!
Laser safety at the LCI facility

 

Guidlines

Guidelines LCI Unit

The LCI facility guidelines must be followed by all users.

 

Nikon Confocal

Tweety

 

Specifications:

- Ti-E inverted point scanning confocal microscope A1+
- Galvanometric scanner
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um

Lasers Dichroic Mirrors Emission Filters

405 diode
488 diode
561 diode
640 diode

405/488
405/488/561
405/488/561/640
BS 20/80

450/50
525/50
595/50
700/75

Nikon Confocal with resonant scanner

Pegasus

Specifications:

- Ti-E inverted point scanning confocal microscope A1R Si
- Galvanometric and resonant scanner
- 2x GaAsP Detectors (green and red),
- 1x32 channel Spectral Detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um

Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon Confocal with resonant scanner, spectral detector and FLIM

Dragon

  

Specifications:

- Ti-E inverted point scanning confocal microscope A1R Si
- Galvanometric and resonant scanner
-  2x GaAsP detectors (green and red),
- 1x32 channel Spectral Detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z
- La Vision BioTech FLIM (Fluorescence Live time Microscopy)

Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon Confocal with spectral detector and STORM

Orion

Specifications:

- Ti-E inverted point scanning confocal microscope A1 Si
- Galvano scanner
- 1x32 channel Spectral detector
- Full size incubator for life cell imaging
- Motorized XY stage and Z piezo 100 um
- TIRF (Total Internal Reflection Fluorescence microscopy)
- STORM (Stochastic Optical Reconstruction microscopy)
- Andor EM-CCD camera

Lasers Dichroic Mirrors Emission Filters

405 diode
457/488/514 Argon Ion
561 diode
640 diode (300 mWatt)

405/488
405/488/561
405/488/561/640
405-457/514
457/514/561
BS 20/80

450/50
482/35
525/50
540/30
595/50
700/75

Nikon spinning disk confocal and widefield

Victor

Specifications:

Nikon Ti-E spinning disk confocal or widefield microscope
Motorized XY stage
Motorized Z piezo stage and Z piezo 250 um
Full size incubator for life cell imaging

Special features: 2 possible configurations:

Spinning disk confocal

- Andor EM-CCD camera. 512x512 pixels. Pixel size 16 um. QE 95%
- Spinning disk Yokagawa X1, 10,000 rpm

Lasers for spinning disk imaging Dichroic Mirror Emission Filters

405 nm diode
488 nm diode
561 nm diode
640 nm diode

425-480
503-555
580-628
675LP

447/60
512/30 ; 630/100
525/30
525 600 690
607/36
685/40

Widefield microscope

- Andor sCMOS Zyla 4.2+. 2014x2048 pixels. Pixel size 6.45 um. QE 82%
-SpectraX light source (six independently controllable light sources that span the visible spectrum from 380-680nm

LEDs from SpectraX for wide field imaging Dichroic Mirrors Emission Filters
395/25 nm
440/20 nm
475/28 nm
510/25 nm
550/15 nm (or 575/25)
631/28 nm
425-480
503-555
580-628
675LP
447/60
483/32
500LP
525/30
542/27
607/36
630/92
685/40

Zeiss single and multi-photon confocal

Zeuss

Specifications:

- Zeiss point scanning confocal LSM710-NLO
- Upright microscope
- Motorized XY stage and Z
- Full size incubator for live cell imaging
- Spectral detector (descanned)
- Single and Two-photon laser
- Two Non-descanned detectors (Epi and Trans)
- GaAsP ultra sensitive detector

Lasers:
- 405nm diode
- 457nm/488nm/514nm Argon Ion
- 561nm diode
- 633nm diode

Objectives

Permanently available on our NIKON microscopes:

Magnification Type n NA WD mm FOV um
10x Dry 1 0.45 4 1257
20x Dry 1 0.75 1 629
60x Oil 1.55 1.40 0.13 314

Additional objectives on our NIKON microscopes:

Magnification Type n NA WD mm FOV um
40 Dry 1 0.95 0.14 (ring) 314
40 Oil 1.52 1.30 0.2 (ring) 314
60 Oil 1.52 1.49   210
60 Water 1.33 1.2 0.31-0.28 (ring) 210
100 Oil 1.52 1.49   126

Objectives available on Zeiss LSM710

Magnification Type n NA WD mm FOV um
5x Dry 1 0.16 18.5 1700
20x Dry 1 0.6 1.7 425
20x Dipping 1.33 1.00 1.8 425
40x Water 1,33 1.10 0.62 213
63x Water 1.33 1.20 0.28 135

Software

We have 4 offline stations at the LCI Facility equipped with the following softwares:

- NIS Element: offline software for the Nikon systems
- Zeiss Zen: offline software for Zeus
- Imspector Pro: offline software for FLIM
- Imaris: powerful 5D rendering and statistical analysis software
- Cell Profiler: 4D (no z) rendering and statistical analysis freeware
- ImageJ/Fiji: image processing and visualizing freeware
- XUV Tools: 3D image alignment freeware
- MatLab: custom-made analysis

Microscopy