About LCI

The Live Cell Imaging Facility is a light microscopy facility at the Department of BioNut in Huddinge

The Live Cell Imaging facility was started in 2009. Since then the facility has grown to include 6 high-end microscopes, 3 experienced staff and a high image analysis capability. About 70 researchers actively use the equipement at the LCI facility.

Together our microscopes allow researchers to perform conventional and high throughput confocal and widefield microscopy, fast confocal microscopy (resonance scanner and spinning disk), Fluorescence LifeTime Imaging (FLIM), multiphoton microscopy, spectral imaging and super resolution microscopy (TIRF and STORM). Additionally the NIS-Element and Imaris software are available for manual or fully automated or semi-automated image processing and analysis.

The Live Cell Imaging facility is grateful to the founding agencies that allowed its development: Vetenskapsrådet, the Knut and Alice Wallenberg Foundation, the Center for Biosciences/CIMED and the KI fund for core facilities.

Today the LCI facility is proud of being part of the The Jonasson Centre for Medical Imaging, the network of Nikon Center of Excellence, as well as being member of several international imaging networks (Swedish Bioimaging Network, European Light Microscopy Initiative).

Jonasson Centre for Medical Imaging and Nikon Center of Excellence

Inauguration of Nikon Center of Excellence 2014

The Jonasson Centre for Medical Imaging was established in 2014 after a donation from Kerstin och Rune Jonasson. The overall aim of the Centre is for healthcare, technology and research in the region to become world-leading within image function.

The LCI facility is happy to host 2 high-end microscopes that are part of the Jonasson Centre: The Pegasus and Orion systems allow researchers to perform confocal imaging and super-resolution microscopy.

In 2014, the LCI facility was awarded the prestigious Nikon Center of Excellence label. The facility became one of only 8 Centers of Excellence in Europe, 15 in the world. The Center officialises a tripartite collaboration between the Live Cell Imaging facility at the Karolinska Institute in Huddinge, the School of Technology and Health at the Royal School of Technology (KTH) in Huddinge and Nikon Instruments Europe.

This collaboration includes sharing equipment, resources and knowledge in order to offer the best possible service to the users of the LCI facility and contribute to the development of the Nikon confocals and software. The Nikon Center of Excellence comprises the 5 state-of-the-art Nikon confocals present at the LCI facility.

Contact

Manager

Research engineer

Sylvie Le Guyader

Phone: +46-(0)8-524 811 07
Organizational unit: Department of Biosciences and Nutrition (BioNut), H2
E-mail: Sylvie.Le.Guyader@ki.se

LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008

Microscopy Engineer

Project coordinator

Gabriela Imreh

Phone: +46-(0)8-524 811 75
Organizational unit: Strömblad
E-mail: Gabriela.Imreh@ki.se

Microscopy Engineer

Assistant Professor

Tobias Nyberg

Phone: +46-8-790-4865

E-mail: tobias.nyberg@sth.kth.se

Director

Professor

Staffan Strömblad

Phone: +46-(0)8-524 811 22
Organizational unit: Strömblad
E-mail: Staffan.Stromblad@ki.se

Address

Novum
Live Cell Imaging facility
Dept. of Biosciences and Nutrition
Karolinska Institutet
141 83 Huddinge, Sweden
(Visit: Novum, Hälsovägen 7, G lift to floor 6)

How to find the LCI Facility

Way to LCI unit

Driving:
Live Cell Imaging Facility
Karolinska Institutet
Dept. of Biosciences and Nutrition
Hälsovägen 7, G lift, floor 6
141 83 Huddinge, Sweden

Public Transport:
- From T-Centralen, take the commuter train (Pendeltåg, indicated by a blue J) towards Tumba or Södertälje C
- Get off at the Flemingsberg station (NOT Huddinge) and walk towards the front of the train.
- Follow the crowd towards the hospital up a small escalator then to the right then up a long escalator.
- At the top of the escalator, walk straight ahead but very slightly to the right, pass the ‘door’ sculpture then below a building and across a small bridge.
- Walk straight ahead for about 200 m and you will see a sign Novum above the path.
- By the sign, walk into the building to your right.
- At the end of the hall on the left is the G lift.
- Go up to the 6th floor then to the glass door just next to the lift.

How to find the Red seminar room:
- Enter the Novum building from the Blickagången 6 side
- Take the stairs (or the E lift) up to the 6th floor
- Enter the BioNut department and take the first corridor to your left. The seminar room will be a few doors away on your right

How to find the Green seminar room:

- Enter the Novum building from the Blickagången 6 side
- At the end of the hall on the right is the F lift.
- Go up to the 6th floor then to the glass door just next to the lift
- Take the second door on your left

Microscopy